FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900102
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT43190.22730358686007382No Hit
GTATTACTGTCAGCACTATCGTACCTCATTCACTTTCGGCCCTGGGACCA42930.22593523926610257No Hit
GTAATAGATGGCGGTGTCCGAGGCCCTCAGGGTGCTCCAGTGAAGGTAGG42660.224514262918517No Hit
ATCGTACCTCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA41420.21798829747034631No Hit
ATCTATTACTGTGCGACACTAGATTACTACTACTATTACGGTATGGACGT38590.20309436019750518No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT28660.1508340078585255No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT28060.1476762826416687No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25190.13257183035437045No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24160.12715106873209966No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23840.1254669486164427No Hit
GTGTTAGTAGCGTCTACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCT23470.12351968473271434No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC23350.12288813968934298No Hit
GTCTGGAGCAGAGGTGAAAAAGCCCGGGGAGTCTCTGAAGATCTCTTGTA23180.12199345087790024No Hit
AGGTACGATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGT21260.11188873018395855No Hit
CTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT19440.10231029702615965No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT19290.10152086572194545No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)