FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009609
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTACTGTGCGAGTTCGTCAGTGGCTGGCACCAGGGTAGGTCTTCT61090.30398948253117897No Hit
GTAATACATAGCCGTGTCAGCGGCGGTCACGGAGCTCACCGTCAGGGAGA51770.2576123016964992No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36850.18336900362209763No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT35220.1752579730683929No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA29320.14589902811940034No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29240.14550094073026146No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29160.14510285334112258No Hit
GTATTACTGTGCGAGTTCGTCAGTGGCTGGCACCAGGGTAGGTCTTCTTT28670.14266456808264694No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC27340.136046365238213No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26010.1294281623937791No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25700.12788557376086593No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG24130.12007310874901536No Hit
ACTACAGCCCGTCCCACAGGGGTCGAGTCACCATAGTGGAAGACACGTCC23420.11654008317040779No Hit
GCTATGTATTACTGTGCGAGTTCGTCAGTGGCTGGCACCAGGGTAGGTCT23170.11529606007934876No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23100.11494773361385224No Hit
ACCTACTACAGCCCGTCCCACAGGGGTCGAGTCACCATAGTGGAAGACAC22940.11415155883557448No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT22160.11027020679147037No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC21800.1084788135403454No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21650.10773239968571002No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21110.10504530980902255No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33000.073.563521
GGTATCA15100.070.127281
TCAACGC39300.060.645464
ATCAACG39600.060.3678553
CAACGCA42350.056.2764325
TATCAAC43400.054.914912
AACGCAG44500.053.3936
CGCAGAG51550.046.230628
ACGCAGA59000.040.148967
GCAGAGT59950.039.7529349
GTACATG48350.032.3303031
TACATGG47950.030.933612
ACATGGG47400.030.2299583
CAGCGCT11400.029.6840679
ATAGCCG28900.029.1486478
TAGCCGT30000.027.8398659
GTAATAC33800.026.2141021
GTTAGGA17900.025.7557071
CAGAGTA63250.024.5880229
TACATAG35900.024.4690675
Produced by FastQC (version 0.11.5)