FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866196
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCAAGTCGCAGGCTGTTCATTTGCAAATGCA98830.529579958375219No Hit
GTATATTACTGTGTGAGAGATCACTGGGGGACCTGGGGCCTGGGATCCCT87380.4682252024974869No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG52510.281374518003468No Hit
ATATACAGCCGTGTCCTCAAGTCGCAGGCTGTTCATTTGCAAATGCACAC46340.24831261025101328No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT43750.23443411088653068No Hit
GCTGTATATTACTGTGTGAGAGATCACTGGGGGACCTGGGGCCTGGGATC42320.22677146451926805No Hit
ATATTACTGTGTGAGAGATCACTGGGGGACCTGGGGCCTGGGATCCCTGG37940.20330126096079942No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT36320.19462050074054385No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36160.19376314170644454No Hit
GAGTATTAGTAATTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGTCC35360.189476346535948No Hit
GATAAATACTACGCAGACTCTGTGGAGGGCCGATTCACCATCTCCAGAGA34590.18535030618434506No Hit
GTTCTTGGAGTTGTCTCTGGAGATGGTGAATCGGCCCTCCACAGAGTCTG34350.18406426763319608No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC34060.18251030438389107No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32810.1758121869299902No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC30880.16547029358116727No Hit
CAGTAATATACAGCCGTGTCCTCAAGTCGCAGGCTGTTCATTTGCAAATG30150.16155859298808914No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA29270.15684311830054293No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA29260.15678953336091173No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28320.15175254903557825No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG27540.1475729237443441No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC26150.14012461713560634No Hit
GTATTTATCAGTCTCAACATATGATATAGTTGCCACCCACTCCAGCCCCT25960.1391065032826134No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGAGTATTAGTAA24760.13267631052686857No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC24470.13112234727756356No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG24220.12978272378678338No Hit
CTCCAAGAACAGTGTGCATTTGCAAATGAACAGCCTGCGACTTGAGGACA23850.12780008102042872No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT23570.12629970271075494No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23340.12506724909923717No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23290.12479932440108113No Hit
AGTTTAGAAAGTGGGGTCCCATCAAGGTTCACCGGCAGTGGATCTGGGAC22830.1223344171780456No Hit
CTATATCATATGTTGAGACTGATAAATACTACGCAGACTCTGTGGAGGGC22820.1222808322384144No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCG22660.1214234732043151No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT22500.12056611417021579No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAACTGGGTTTTCCTCGT22230.11911932080017318No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG22170.11879781116238594No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22060.11820837682644267No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG21630.11590422442230076No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21540.1154219599656199No Hit
ATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG21520.11531479008635749No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21460.11499328044857024No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21240.1138144117766837No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG21200.11360007201815887No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA21040.11274271298405956No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20420.10942044672692472No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC20160.10802723829651333No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGA19940.10684836962462678No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT19740.10577667083200265No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA19060.10213289493708057No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18960.1015970455407685No Hit
GTATTAGTAATTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGTCCCT18900.10127553590298126No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG18850.10100761120482521No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31550.073.755381
ATCAACG38950.058.9792333
TCAACGC39700.057.865024
CAACGCA40750.056.3715
TATCAAC41950.055.2749252
AACGCAG42300.054.3010256
CGCAGAG47550.048.4544758
GCAGAGT55800.040.9034089
GTACATG49700.036.2388271
TACATGG49700.035.788612
GTAATAT43700.035.4443931
CAGCGCT10050.035.1046379
ACATGGG49450.034.6598433
AATATAC46500.033.1418463
CATGGGA26600.032.4874424
CAGCCGT46100.032.3298659
GAATACG4050.032.000149
TATACAG50150.031.7332065
TAATATA50050.030.9341812
ATATACA55600.029.6603724
Produced by FastQC (version 0.11.5)