FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866196
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCAAGTCGCAGGCTGTTCATTTGCAAATGCA89680.48054973861266453No Hit
GTATATTACTGTGTGAGAGATCACTGGGGGACCTGGGGCCTGGGATCCCT76760.41131799660914503No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG48070.2575828048072121No Hit
ATATACAGCCGTGTCCTCAAGTCGCAGGCTGTTCATTTGCAAATGCACAC42260.22644995488148084No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT40990.21964466754831755No Hit
GCTGTATATTACTGTGTGAGAGATCACTGGGGGACCTGGGGCCTGGGATC37040.19847861639399078No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT34350.18406426763319608No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33010.17688388572261435No Hit
ATATTACTGTGTGAGAGATCACTGGGGGACCTGGGGCCTGGGATCCCTGG32730.17538350741294056No Hit
GTTCTTGGAGTTGTCTCTGGAGATGGTGAATCGGCCCTCCACAGAGTCTG30770.16488085924522397No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30680.1643985947885431No Hit
GAGTATTAGTAATTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGTCC29750.15941519540284085No Hit
GATAAATACTACGCAGACTCTGTGGAGGGCCGATTCACCATCTCCAGAGA29010.15544990987013152No Hit
CAGTAATATACAGCCGTGTCCTCAAGTCGCAGGCTGTTCATTTGCAAATG28830.1544853809567698No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC27600.14789443338213135No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26460.14178575026417375No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA25840.13846348400703892No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24860.1332121599231806No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC24240.12988989366604578No Hit
GTATTTATCAGTCTCAACATATGATATAGTTGCCACCCACTCCAGCCCCT24200.12967555390752097No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23070.12362045572919457No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC22820.1222808322384144No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22720.12174498284210233No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21920.11745818767160576No Hit
AGTTTAGAAAGTGGGGTCCCATCAAGGTTCACCGGCAGTGGATCTGGGAC21810.1168687533356625No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21770.11665441357713766No Hit
CTATATCATATGTTGAGACTGATAAATACTACGCAGACTCTGTGGAGGGC21720.11638648887898162No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGAGTATTAGTAA21190.11354648707852767No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT21170.11343931719926524No Hit
CTCCAAGAACAGTGTGCATTTGCAAATGAACAGCCTGCGACTTGAGGACA20920.11209969370848506No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20690.1108672400969673No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA20640.11059931539881127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20630.11054573045918006No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20320.10888459733061265No Hit
TTCTAAACTAGTCGCCTGATAGATCAGGAGCTTAGGGACTTTCCCTGGTT20190.10818799311540697No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCG20100.10770572865872609No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAACTGGGTTTTCCTCGT20010.10722346420204522No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC19770.10593742565089626No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG18980.10170421542003091No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC18980.10170421542003091No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG18850.10100761120482521No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT18840.10095402626519401No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31400.075.209251
ATCAACG38600.060.25283
TCAACGC38850.060.050414
TATCAAC41050.056.4813042
CAACGCA43250.054.274215
AACGCAG45500.051.590326
CGCAGAG48700.047.904698
GCAGAGT56000.041.7818349
GTACATG49100.036.3661461
TACATGG49950.034.452792
ACATGGG51450.033.1684343
CATGGGA29250.031.2636624
GTAATAT43750.030.2807661
CAGCCGT44500.029.7680239
TATACAG47650.029.617965
AATATAC45550.029.5607343
TCCGACC1952.7707392E-729.540478
GTACACG18550.029.112495
TACACGG18600.029.0342316
GTATGTA23600.028.372531
Produced by FastQC (version 0.11.5)