FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742244
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA27360.3686119389311332No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC22160.2985541142804792No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATCGTAACACCAATCGGCCCT19880.26783645270288475No Hit
GTATGTGCTACTTCCACCTCTATAGATAACTGAGACCCACTCCAGGCCCT18190.24506765969142222No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16750.22566703132662577No Hit
GTTTATTACTGTGCGAAAGGTAGTGACTACGGTGACTACGTCACTCACTG15710.21165546639649496No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC14980.20182042562823005No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA14890.20060788635543028No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCCCCCCACTCAGG14810.19953007366849715No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14750.1987217141532973No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTCTGATGTTCACT14580.19643136219356436No Hit
AGCACATACTATGCGGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA14480.19508409633489796No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCCC13800.1859226884959663No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG13650.18390178970796664No Hit
TCCTATGACAGCAGCCTGAGTGGGGGGGTCTTCGGCGGAGGGACCAAGCT13570.1828239770210335No Hit
GTCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTG13290.17905163261676754No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13050.17581819455596812No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12820.17271948308103535No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12520.1686776855050361No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12210.16450116134317017No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12070.1626149891410372No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11680.15736065229223814No Hit
TCGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCA11400.15358830788797215No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11320.152510495201039No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11220.15116322934237258No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10720.1444269000490405No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA10620.14307963419037406No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT10300.1387683834426415No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10220.13769057075570834No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10030.13513076562424217No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9900.1333793200079758No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9880.1331098668362425No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC9750.13135842121997618No Hit
ACTCAGGACAGTCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCC9580.12906806926024328No Hit
GGACTGAACAGAGAGGACTTACCATGGAGTTTGGGCTGAGCTGGCTTTTT9310.12543045144184392No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9240.12448736534077744No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9120.12287064631037771No Hit
CTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGACATTGTCGG9050.12192756020931123No Hit
ATCGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCC8920.12017611459304486No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8860.11936775507784501No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8790.11842466897677853No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCCCCCCACTCAGGC8780.11828994239091188No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG8690.11707740311811211No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA8640.1164037701887789No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGGGGGGTCTTCGG8610.11599959043117895No Hit
CTATAGAGGTGGAAGTAGCACATACTATGCGGACTCCGTGAAGGGCCGGT8560.11532595750184575No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA8540.11505650433011247No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC8540.11505650433011247No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8510.11465232457251254No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8510.11465232457251254No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8480.11424814481491262No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA8340.11236197261277964No Hit
GTGTTACGATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGGTGCTGGTA8300.11182306626931306No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8170.11007162065304671No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGTTGTTGGAGTCTGGGGGAGGCT8120.10939798772371351No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8100.10912853455198022No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8080.10885908138024691No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG8030.10818544845091371No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC7990.10764654210744715No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7970.10737708893571386No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGT7870.10602982307704745No Hit
TACCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCATCTATCGTAACAC7790.10495201039011431No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT7780.10481728380424765No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7780.10481728380424765No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7700.10373947111731452No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7660.10320056477384795No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7650.10306583818798132No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTACCGACAATGTCATGAGCTGGG7600.1023922052586481No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT7590.10225747867278147No Hit
CTATGCGGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGATAATTCCA7530.10144911915758159No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT7470.10064075964238174No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG7450.10037130647064846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC12500.055.8861734
ATCAACG13100.053.876263
CAACGCA13600.051.365975
AACGCAG13650.051.1674656
TATCAAC13750.050.2852362
GAATACG3800.049.2590529
CGCAGAG14400.048.495968
GTATCAA16250.043.881661
GCAGAGT17250.040.483589
TCTACAC7550.040.0631453
GGTATCA6150.039.8203471
GTGCTAG4750.039.425841
GCTAGAG4700.038.3075873
CTAGAGA4800.037.5095144
GGCAGCG3700.036.9748767
ACGCAGA19950.035.0093237
ACACAGT10200.033.178186
AGAATAC5650.033.1299828
ACAGTGA9650.032.826288
TAATAGT2009.544237E-932.4082224
Produced by FastQC (version 0.11.5)