FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999072
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCATTGATGGCCAGGGAGG37630.3766495307645495No Hit
GATTATTACTGTGCAACATGGGATGACAAACTGAATGGTTTTGTCTTCGG35430.35462909580090324No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGCCTCCTCAGTTCCATGTAGG27080.2710515358252458No Hit
CCTCATATATTATGATGATCTACTGCCCGCAGGGGTCTCTGACCGATTCT24420.24442682809647354No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCT21630.21650091284712214No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGCAGCCC21480.21499951955414623No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC18220.18236923865347043No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17690.17706431568495565No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17680.17696422279875726No Hit
TCCTCACCCTCATCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG17450.1746620864161942No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT17420.17436180775759905No Hit
GTGTATTACTGTGCGACTTGGGCGGGACCAGAGCAGGGAAGGGACGCTTT17220.1723599500336312No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA16980.1699577207648698No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16580.16595400531693413No Hit
GTCTGGAACTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA16020.16034880368982415No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15800.15814676019345952No Hit
ACCTAGGACGGTGACCTTGGTCCCAGGTCCGAAGACAAAACCATTCAGTT15570.1558446238108965No Hit
GACCTAGGACGGTGACCTTGGTCCCAGGTCCGAAGACAAAACCATTCAGT15190.1520410941353576No Hit
ATATTATGATGATCTACTGCCCGCAGGGGTCTCTGACCGATTCTCTGGCT15040.15053970084238172No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14920.14933858620800103No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14900.14913840043560422No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT14830.1484377502322155No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14360.14373338458089105No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14090.14103087665353448No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC13770.13782790429518593No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13700.13712725409179718No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC13640.13652669677460683No Hit
CATCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC13240.13252298132667117No Hit
GTGCTGACTCAGCCACCCTCGGTGTCTGCAGCCCCCAGGCAGAGGGTCAC13010.13022084494410813No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12750.12761842990294994No Hit
GCCTACATGGAACTGAGGAGGCTGAGATCTGACGACACGGCCGTGTATTA12680.1269177796995612No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12620.12631722238237084No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12460.12471573620319656No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG11480.11490663335575414No Hit
TATATGAGGAGCTTCGGAGCCCTTCCTGGGAGTTGCTGGTACCACTTCAC11400.11410589026616699No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11190.11200393965600076No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG11190.11200393965600076No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10730.10739966689087474No Hit
TCCCTGCTCTGGTCCCGCCCAAGTCGCACAGTAATACACGGCCGTGTCGT10700.10709938823227955No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10590.10599836648409725No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10010.1001929790845905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18250.068.3689961
GGTATCA7750.067.004931
ATCAACG21350.057.0223
TCAACGC21350.057.0224
CAACGCA21750.055.9705125
AACGCAG22750.053.472786
TATCAAC22950.053.3604932
CGCAGAG26600.045.7310038
ACGCAGA28350.042.9081047
GTACTAA1001.4347337E-436.061681
GTACATG29150.034.886431
CAGCGCT5600.033.418819
GAATACG1801.3768476E-731.9906569
GCAGAGT43050.028.4237549
ACCGTCG1050.007984552527.4205658
GTACACG10150.026.9680425
GCAGCGC7450.026.0863428
GTAATAC15900.025.8555431
TATGTAC11200.025.727382
ATCAGCC25000.025.6245167
Produced by FastQC (version 0.11.5)