FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999072
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCATTGATGGCCAGGGAGG36070.3610350405176003No Hit
GATTATTACTGTGCAACATGGGATGACAAACTGAATGGTTTTGTCTTCGG32640.32670318055155184No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGCCTCCTCAGTTCCATGTAGG25090.2511330514717658No Hit
CCTCATATATTATGATGATCTACTGCCCGCAGGGGTCTCTGACCGATTCT21900.21920342077447874No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGCAGCCC19660.19678261426603888No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCT19330.19347954902149195No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT16910.16925707056148104No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA16260.1627510329585856No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16220.162350661413792No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15930.15944796771403863No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15890.15904759616924505No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC15750.15764629576246758No Hit
GTGTATTACTGTGCGACTTGGGCGGGACCAGAGCAGGGAAGGGACGCTTT15540.15554434515230134No Hit
TCCTCACCCTCATCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG15490.15504388072130937No Hit
GTCTGGAACTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA14460.144734313442875No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14440.1445341276704782No Hit
GACCTAGGACGGTGACCTTGGTCCCAGGTCCGAAGACAAAACCATTCAGT14310.1432329201498991No Hit
ATATTATGATGATCTACTGCCCGCAGGGGTCTCTGACCGATTCTCTGGCT14280.14293264149130394No Hit
ACCTAGGACGGTGACCTTGGTCCCAGGTCCGAAGACAAAACCATTCAGTT13980.13992985490535215No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13370.13382418884725023No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13250.13262307421286954No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT13080.13092149514749687No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12840.12851926587873547No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12590.12601694372377567No Hit
CATCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC12570.1258167579513789No Hit
GTGCTGACTCAGCCACCCTCGGTGTCTGCAGCCCCCAGGCAGAGGGTCAC11890.11901044168988821No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC11870.11881025591749143No Hit
TATATGAGGAGCTTCGGAGCCCTTCCTGGGAGTTGCTGGTACCACTTCAC11310.11320505429038147No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC11070.11080282502162006No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10940.10950161750104097No Hit
GCCTACATGGAACTGAGGAGGCTGAGATCTGACGACACGGCCGTGTATTA10940.10950161750104097No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10930.10940152461484257No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10680.10689920245988277No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10380.10389641587393102No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG10380.10389641587393102No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10310.10319576567054227No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA10210.10219483680855834No Hit
TCATAATATATGAGGAGCTTCGGAGCCCTTCCTGGGAGTTGCTGGTACCA10130.10139409371897119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12900.056.91151
ATCAACG12900.055.1767583
TCAACGC13950.051.0287864
CAACGCA14150.050.825875
AACGCAG14750.049.253366
GGTATCA6750.046.917891
TATCAAC15450.046.0699122
CGCAGAG17150.043.199598
TACCGTA951.0778829E-437.8575869
ACGCAGA20550.035.352177
GTACACG8250.033.1261445
CAGCGCT5100.032.4387559
GTACATG27100.032.402621
ATACACG14700.031.794374
GTATGTA11300.031.2109621
TATGTAC9300.030.9234372
TATTACG1402.8989885E-530.826897
GTAATAC16050.030.4946331
GAATACG1754.014084E-628.7717659
ACACGGG9600.028.472067
Produced by FastQC (version 0.11.5)