FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599445
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAGATGTCAGGCTGCTCAGCTCCATGTAGG29930.4992951813761063No Hit
TTCCTATCCTTGGTATACCACACAACGCACAGAAGTTCCAGGGCAGAGTC20530.3424834638707471No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCAGTGAAGGTCTCCTGCA18380.30661695401579797No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16830.28075970272502065No Hit
GCCTACATGGAGCTGAGCAGCCTGACATCTGAGGACACGGCCGTGTATTA16700.27859103003611674No Hit
GTGTATTACTGTGCGAGACGCGGCTCCTATGATAGTAGTGGTTATTACGA15910.2654121729266238No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15800.26357714218985895No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14480.24155677334868086No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14110.23538439723410823No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13810.23037976795202228No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13210.2203705093878504No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGAGGGATCATTCCTATCCTTGGT13010.21703408986645983No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12800.21353084936899966No Hit
GGATAGGAATGATCCCTCCCATCCACTCAAGCCCCTGTCCAGGGGCCTGT12240.2041888747091059No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11930.19901742445095047No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG11500.19184412247996063No Hit
ATACCACACAACGCACAGAAGTTCCAGGGCAGAGTCACGGTTACCGCGGA11400.19017591271926532No Hit
CATGTAGGCTGTGCTCGTGGGTTTGTCCGCGGTAACCGTGACTCTGCCCT11150.18600538831752705No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG10820.18050029610723253No Hit
TATACAAGCAGCACCCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGT10810.180333475131163No Hit
GTCCAGGGGCCTGTCGCACCCAGTTGATATTATGACCGCTGAAGGTGCCT10760.17949937025081533No Hit
CCATGTAGGCTGTGCTCGTGGGTTTGTCCGCGGTAACCGTGACTCTGCCC10710.17866526537046767No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10370.1729933521841036No Hit
CTACTATCATAGGAGCCGCGTCTCGCACAGTAATACACGGCCGTGTCCTC10150.16932329071057395No Hit
GCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC9850.16431866142848803No Hit
ATCATAGGAGCCGCGTCTCGCACAGTAATACACGGCCGTGTCCTCAGATG9820.16381819850027943No Hit
CTCCTGGACAGTCGATCACCGTCTCCTGCACTGGAAGCAGCAGTGACCTT9690.16164952581137554No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9690.16164952581137554No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9670.16131588385923645No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9610.16031495800281928No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC9380.15647807555322005No Hit
CAGTAATACACGGCCGTGTCCTCAGATGTCAGGCTGCTCAGCTCCATGTA9140.1524743721275513No Hit
GTATACCACACAACGCACAGAAGTTCCAGGGCAGAGTCACGGTTACCGCG9070.1513066252950646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.14980523651043884No Hit
GATTATTACTGCAGCTCATATACAAGCAGCACCCCTTGGGTGTTCGGCGG8890.14830384772581304No Hit
GTATTACTGTGCGAGACGCGGCTCCTATGATAGTAGTGGTTATTACGAGA8870.14797020577367398No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8850.14763656382153492No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8820.14713610089332632No Hit
GTGTGGTATACCAAGGATAGGAATGATCCCTCCCATCCACTCAAGCCCCT8790.14663563796511772No Hit
ATACACGGCCGTGTCCTCAGATGTCAGGCTGCTCAGCTCCATGTAGGCTG8700.14513424918049195No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGG8660.14446696527621383No Hit
TTGGTATACCACACAACGCACAGAAGTTCCAGGGCAGAGTCACGGTTACC8610.14363286039586617No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8540.14246511356337946No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8540.14246511356337946No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8500.14179782965910134No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT8420.14046326185054508No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8380.13979597794626697No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8280.13812776818557165No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT8240.13746048428129354No Hit
AGCTACAGGTGTCCAGTCCCAGGTCCAGCTGGTGCAGTCTGGGGCTGAGG8030.13395724378383336No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT7890.13162175011885996No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7820.13045400328637322No Hit
CATAGGAGCCGCGTCTCGCACAGTAATACACGGCCGTGTCCTCAGATGTC7820.13045400328637322No Hit
CCCAAGGGGTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCC7820.13045400328637322No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7640.12745122571712167No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7630.12728440474105215No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA7630.12728440474105215No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG7510.12528255302821778No Hit
GTCCCAGGTCCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGT7360.1227802383871748No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC7340.12244659643503575No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG7290.12161249155468808No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7250.12094520765040997No Hit
TGATAGTAGTGGTTATTACGAGATCGACTCCTGGGGCCAGGGAACCCTGG7240.12077838667434043No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG7190.11994428179399277No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT7170.1196106398418537No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC7160.11944381886578419No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC7130.1189433559375756No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7080.11810925105722794No Hit
GCTACAGGTGTCCAGTCCCAGGTCCAGCTGGTGCAGTCTGGGGCTGAGGT7030.11727514617688027No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA7000.11677468324867168No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT6990.11660786227260217No Hit
GTATACCAAGGATAGGAATGATCCCTCCCATCCACTCAAGCCCCTGTCCA6950.11594057836832403No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC6890.11493965251190685No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG6840.1141055476315592No Hit
GTCATAATATCAACTGGGTGCGACAGGCCCCTGGACAGGGGCTTGAGTGG6780.11310462177514201No Hit
GTGGTATACCAAGGATAGGAATGATCCCTCCCATCCACTCAAGCCCCTGT6750.11260415884693342No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT6670.11126959103837718No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT6640.11076912811016858No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC6630.11060230713409905No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC6630.11060230713409905No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG6620.1104354861580295No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG6590.1099350232298209No Hit
GTACATGGGACATAACAACCAGATTCCTCCTCTAAAGAAGCCCCTGGGAG6530.10893409737340373No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6490.10826681346912562No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA6480.10809999249305607No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG6450.10759952956484749No Hit
GCCGTGTATTACTGTGCGAGACGCGGCTCCTATGATAGTAGTGGTTATTA6430.10726588761270843No Hit
TCCTATGATAGTAGTGGTTATTACGAGATCGACTCCTGGGGCCAGGGAAC6410.10693224566056936No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC6390.1065986037084303No Hit
GATTATAGCTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGGCCCCAA6280.10476357297166546No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA6280.10476357297166546No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG6180.10309536321097014No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6180.10309536321097014No Hit
CGTAATAACCACTACTATCATAGGAGCCGCGTCTCGCACAGTAATACACG6080.10142715345027485No Hit
CCCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTTCGTCAGCCC6070.10126033247420531No Hit
ACATGGAGCTGAGCAGCCTGACATCTGAGGACACGGCCGTGTATTACTGT6060.10109351149813577No Hit
CACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA6050.10092669052206625No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12900.065.321551
ATCAACG13300.061.175633
GGTATCA6150.060.8959661
TCAACGC13550.060.0469254
AACGCAG14650.055.538286
CGCAGAG16500.049.307158
GCGCTAC450.00990590347.998117
TATTGAG7200.041.0018045
TTATTGA7250.040.7190324
ACGCAGA21900.037.149227
CATGGGG7650.035.7662774
GTACATG15200.034.5891271
ATTGAGG3350.034.3895726
ATTGAGT5850.033.2322356
ACATGGG15700.033.020563
ATTACAC1751.0708027E-732.9157333
GTCTTAT900.0037466932.0094221
GGTTTAT5400.032.009421
ACCCTTA900.00375281831.9987417
ATGGGAC4750.031.8329815
Produced by FastQC (version 0.11.5)