FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599445
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAGATGTCAGGCTGCTCAGCTCCATGTAGG33880.565189466923571No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20560.3429839267989557No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCAGTGAAGGTCTCCTGCA18050.3011118618055034No Hit
GCCTACATGGAGCTGAGCAGCCTGACATCTGAGGACACGGCCGTGTATTA16650.2777569251557691No Hit
TTCCTATCCTTGGTATACCACACAACGCACAGAAGTTCCAGGGCAGAGTC16570.2764223573472128No Hit
GTGTATTACTGTGCGAGACGCGGCTCCTATGATAGTAGTGGTTATTACGA16330.2724186539215441No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15410.25707112412314725No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14650.2443927299418629No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14530.24239087822902852No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13860.23121387283236997No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12950.21603316401004263No Hit
GTCCAGGGGCCTGTCGCACCCAGTTGATATTATGACCGCTGAAGGTGCCT12440.20752529423049654No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12310.20535662154159265No Hit
GGATAGGAATGATCCCTCCCATCCACTCAAGCCCCTGTCCAGGGGCCTGT11630.19401279516886452No Hit
CCATGTAGGCTGTGCTCGTGGGTTTGTCCGCGGTAACCGTGACTCTGCCC11610.19367915321672546No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGAGGGATCATTCCTATCCTTGGT11520.1921777644320997No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11510.19201094345603015No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG11360.1895086288149872No Hit
CATGTAGGCTGTGCTCGTGGGTTTGTCCGCGGTAACCGTGACTCTGCCCT11000.18350307367648408No Hit
CTACTATCATAGGAGCCGCGTCTCGCACAGTAATACACGGCCGTGTCCTC10970.18300261074827548No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10850.1810007590354411No Hit
TATACAAGCAGCACCCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGT10580.17649659268156379No Hit
ATCATAGGAGCCGCGTCTCGCACAGTAATACACGGCCGTGTCCTCAGATG10490.17499520389693798No Hit
CAGTAATACACGGCCGTGTCCTCAGATGTCAGGCTGCTCAGCTCCATGTA10340.17249288925589504No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG10090.16832236485415678No Hit
ATACACGGCCGTGTCCTCAGATGTCAGGCTGCTCAGCTCCATGTAGGCTG9680.161482704835306No Hit
GTATTACTGTGCGAGACGCGGCTCCTATGATAGTAGTGGTTATTACGAGA9660.16114906288316694No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9640.16081542093102785No Hit
GATTATTACTGCAGCTCATATACAAGCAGCACCCCTTGGGTGTTCGGCGG9550.15931403214640208No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9520.15881356921819348No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9520.15881356921819348No Hit
GCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC9500.15847992726605442No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9450.1576458223857068No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC9410.15697853848142865No Hit
CTCCTGGACAGTCGATCACCGTCTCCTGCACTGGAAGCAGCAGTGACCTT9180.15314165603182944No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9170.1529748350557599No Hit
ATACCACACAACGCACAGAAGTTCCAGGGCAGAGTCACGGTTACCGCGGA9070.1513066252950646No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8730.14563471210870055No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8390.13996279892233648No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8270.1379609472095021No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8230.137293663305224No Hit
CCCAAGGGGTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCC8180.13645955842487634No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8170.1362927374488068No Hit
CATAGGAGCCGCGTCTCGCACAGTAATACACGGCCGTGTCCTCAGATGTC8020.13379042280776385No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT7980.13312313890348573No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7950.13262267597527713No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT7900.13178857109492947No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGG7880.13145492914279042No Hit
GTGTGGTATACCAAGGATAGGAATGATCCCTCCCATCCACTCAAGCCCCT7820.13045400328637322No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC7820.13045400328637322No Hit
AGCTACAGGTGTCCAGTCCCAGGTCCAGCTGGTGCAGTCTGGGGCTGAGG7810.1302871823103037No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7790.12995354035816462No Hit
GTATACCACACAACGCACAGAAGTTCCAGGGCAGAGTCACGGTTACCGCG7670.12795168864533024No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT7640.12745122571712167No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT7620.1271175837649826No Hit
TGATAGTAGTGGTTATTACGAGATCGACTCCTGGGGCCAGGGAACCCTGG7560.1261166579085654No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7480.12478209010000918No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG7440.12411480619573105No Hit
GTCCCAGGTCCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGT7290.12161249155468808No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG7250.12094520765040997No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG7120.11877653496150606No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT7110.11860971398543654No Hit
GTGGTATACCAAGGATAGGAATGATCCCTCCCATCCACTCAAGCCCCTGT7100.118442893009367No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7100.118442893009367No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA7030.11727514617688027No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG6960.11610739934439357No Hit
GTATACCAAGGATAGGAATGATCCCTCCCATCCACTCAAGCCCCTGTCCA6930.11560693641618498No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG6890.11493965251190685No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT6860.11443918958369825No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA6850.11427236860762871No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC6810.11360508470335061No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG6800.11343826372728107No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC6790.11327144275121152No Hit
GCTACAGGTGTCCAGTCCCAGGTCCAGCTGGTGCAGTCTGGGGCTGAGGT6720.11210369591872482No Hit
CCCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTTCGTCAGCCC6630.11060230713409905No Hit
TTGGTATACCACACAACGCACAGAAGTTCCAGGGCAGAGTCACGGTTACC6580.10976820225375139No Hit
CGTAATAACCACTACTATCATAGGAGCCGCGTCTCGCACAGTAATACACG6570.10960138127768186No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC6570.10960138127768186No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC6550.1092677393255428No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG6530.10893409737340373No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA6480.10809999249305607No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6400.10676542468449983No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG6400.10676542468449983No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC6390.1065986037084303No Hit
GCCTTGGGCTGACGAAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA6360.1060981407802217No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT6360.1060981407802217No Hit
GTCATAATATCAACTGGGTGCGACAGGCCCCTGGACAGGGGCTTGAGTGG6340.10576449882808264No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG6330.10559767785201311No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC6320.10543085687594357No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6290.10493039394773498No Hit
TCCTATGATAGTAGTGGTTATTACGAGATCGACTCCTGGGGCCAGGGAAC6280.10476357297166546No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA6230.10392946809131781No Hit
GATTATAGCTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGGCCCCAA6150.10259490028276155No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC6120.10209443735455295No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6110.10192761637848344No Hit
CCACTACTATCATAGGAGCCGCGTCTCGCACAGTAATACACGGCCGTGTC6060.10109351149813577No Hit
CCTATGATAGTAGTGGTTATTACGAGATCGACTCCTGGGGCCAGGGAACC6030.10059304856992718No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT6020.10042622759385766No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA6020.10042622759385766No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)