FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694076
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCACCTCTCAGGCTGTTCATTTGCAGATAAA51980.7489093413401414No Hit
GTATATTACTGTGCGACGAGAGGGACGGACCTGGCCCCGGTGACCTCTGA35890.5170903474547456No Hit
ACTGAATACTATGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGA24040.3463597646367257No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAACTGATGGAGTCTGGGGGAGGCG23170.33382511425261785No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21250.30616243754286276No Hit
GTATTCAGTAATTCCATCATATGACAAAACTGCCACCCACTCCAGCCCCT20600.296797468865081No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA19790.2851272771281531No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18940.27288077962643864No Hit
ATATACAGCCGTGTCCTCACCTCTCAGGCTGTTCATTTGCAGATAAAGAG18460.2659651104489998No Hit
GTACTGGAGGTATTCAGAGGTCACCGGGGCCAGGTCCGTCCCTCTCGTCG17560.25299823074130207No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17150.2470910966522398No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16400.23628536356249172No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16040.23109861167941262No Hit
ATACTATGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAATT15190.21885211417769812No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14590.21020752770589965No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14420.20775822820555673No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13420.19335058408589262No Hit
GCTGTATATTACTGTGCGACGAGAGGGACGGACCTGGCCCCGGTGACCTC13330.19205389611512283No Hit
GTTATGCATTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC13330.19205389611512283No Hit
ATGTTATGCATTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG11890.1713068885828065No Hit
CTATGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAATTCCA11670.1681372068764804No Hit
CCTCTCAGGCTGTTCATTTGCAGATAAAGAGTGTTCTTGGAATTGTCTCT11670.1681372068764804No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11620.16741682467049718No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11440.16482344872895763No Hit
GGGCTGGAGTGGGTGGCAGTTTTGTCATATGATGGAATTACTGAATACTA11350.16352676075818787No Hit
CAGTAATATACAGCCGTGTCCTCACCTCTCAGGCTGTTCATTTGCAGATA11290.16266230211100802No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGTTATGCATTGGG11290.16266230211100802No Hit
ATATTACTGTGCGACGAGAGGGACGGACCTGGCCCCGGTGACCTCTGAAT11250.16208599634622145No Hit
GCATAGTATTCAGTAATTCCATCATATGACAAAACTGCCACCCACTCCAG11110.16006892616946847No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAATGCATAACATAGCTAC10460.1507039574916868No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10100.1455172056086077No Hit
GTCCAGTGTCAGGTGCAACTGATGGAGTCTGGGGGAGGCGTTGTCCAGCC10100.1455172056086077No Hit
GTCTGCATAGTATTCAGTAATTCCATCATATGACAAAACTGCCACCCACT9950.1433560589906581No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9750.14047453016672526No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9690.1396100715195454No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9520.13716077201920251No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9490.13672854269561258No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9490.13672854269561258No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC9480.13658446625441595No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT9460.13629631337202266No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG9330.13442331963646634No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT9240.13312663166569655No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGTT9220.13283847878330327No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT9210.13269440234210664No Hit
ATTCAGAGGTCACCGGGGCCAGGTCCGTCCCTCTCGTCGCACAGTAATAT9160.13197402013612342No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA9140.13168586725373013No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACCAGTCAGA8900.12822803266501076No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA8790.1266431918118477No Hit
CTCCAGTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCCTCCAC8760.12621096248825778No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG8740.1259228096058645No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG8710.12549058028227458No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG8670.12491427451748799No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC8640.12448204519389808No Hit
ATGCATAACATAGCTACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTC8600.12390573942911151No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC8500.1224649750171451No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT8470.12203274569355517No Hit
GCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA8250.11886306398722905No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8210.11828675822244249No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA8210.11828675822244249No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC8190.11799860534004922No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC8000.11526115295731304No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT7950.11454077075132983No Hit
ATGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAATTCCAAG7920.1141085414277399No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG7710.11108293616261043No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT7460.1074810251326944No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7450.10733694869149775No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC7420.10690471936790785No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG7340.10575210783833469No Hit
GCCTGAGAGGTGAGGACACGGCTGTATATTACTGTGCGACGAGAGGGACG7310.1053198785147448No Hit
GATAAAGAGTGTTCTTGGAATTGTCTCTGGAGATGGTGAATCGGCCCTCC7260.10459949630876157No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC7230.10416726698517166No Hit
CTCTTTATCTGCAAATGAACAGCCTGAGAGGTGAGGACACGGCTGTATAT7040.10142981460243548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6990.10070943239645226No Hit
GATGTACACTGGTACCAGCAATTTCCAGGAACAGCCCCCAAATTCCTCAT6950.1001331266316657No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGCG502.0805435E-457.5955357
TACACGT700.001093031741.142634
TCAACGC19950.038.977234
ATCAACG20200.038.856873
TACACCG750.001534414138.3997885
CAGCGCT3750.038.3970229
CAGCCGT23250.038.0873689
TATCAAC21150.037.4522
CAACGCA20900.037.2055365
TATACAG25100.036.716935
GTAATAT24600.036.598351
AACGCAG21250.036.592746
AATATAC24850.035.9325453
TACAGCC25250.035.3556757
CGCAGAG22150.035.103378
ATACAGC25550.034.9430546
ATATACA26750.034.4521454
TAATATA25850.034.263942
GCAGCGC4800.032.997448
GCAGAGT24050.032.6294869
Produced by FastQC (version 0.11.5)