FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694076
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCACCTCTCAGGCTGTTCATTTGCAGATAAA51260.7385358375739832No Hit
GTATATTACTGTGCGACGAGAGGGACGGACCTGGCCCCGGTGACCTCTGA35950.5179548061019255No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23600.34002040122407345No Hit
ACTGAATACTATGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGA23600.34002040122407345No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAACTGATGGAGTCTGGGGGAGGCG21480.3094761956903855No Hit
GTATTCAGTAATTCCATCATATGACAAAACTGCCACCCACTCCAGCCCCT20400.29391594004114824No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18860.2717281680968655No Hit
ATATACAGCCGTGTCCTCACCTCTCAGGCTGTTCATTTGCAGATAAAGAG18020.2596257470363476No Hit
GTACTGGAGGTATTCAGAGGTCACCGGGGCCAGGTCCGTCCCTCTCGTCG17770.2560238360064316No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17600.2535745365060887No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15670.2257677833551369No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15240.21957249638368134No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14830.21366536229461905No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14810.21337720941222577No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14700.21179236855906272No Hit
ATACTATGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAATT13740.19796103020418512No Hit
GTTATGCATTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC13410.19320650764469596No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12890.18571453270247062No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12530.18052778081939153No Hit
GCTGTATATTACTGTGCGACGAGAGGGACGGACCTGGCCCCGGTGACCTC12310.1773580991130654No Hit
ATGTTATGCATTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG12210.17591733470109902No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11680.16828128331767703No Hit
GGGCTGGAGTGGGTGGCAGTTTTGTCATATGATGGAATTACTGAATACTA11670.1681372068764804No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGTTATGCATTGGG11440.16482344872895763No Hit
CAGTAATATACAGCCGTGTCCTCACCTCTCAGGCTGTTCATTTGCAGATA11400.16424714296417106No Hit
CTATGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAATTCCA11250.16208599634622145No Hit
ATATTACTGTGCGACGAGAGGGACGGACCTGGCCCCGGTGACCTCTGAAT11250.16208599634622145No Hit
CCTCTCAGGCTGTTCATTTGCAGATAAAGAGTGTTCTTGGAATTGTCTCT10980.15819593243391214No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAATGCATAACATAGCTAC10950.15776370311032223No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10860.15646701513955244No Hit
GCATAGTATTCAGTAATTCCATCATATGACAAAACTGCCACCCACTCCAG10690.15401771563920955No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10670.15372956275681626No Hit
GTCCAGTGTCAGGTGCAACTGATGGAGTCTGGGGGAGGCGTTGTCCAGCC10350.14911911663852373No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10340.14897504019732707No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10080.14522905272621442No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC10020.14436459407903457No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9900.14263567678467487No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGTT9810.1413389888139051No Hit
CTCCAGTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCCTCCAC9530.13730484846039914No Hit
GTCTGCATAGTATTCAGTAATTCCATCATATGACAAAACTGCCACCCACT9450.136152236930826No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9360.13485554896005625No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG9250.1332707081068932No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC9190.13240624945971335No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9170.13211809657732007No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT9130.1315417908125335No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG8860.12765172690022417No Hit
ATTCAGAGGTCACCGGGGCCAGGTCCGTCCCTCTCGTCGCACAGTAATAT8760.12621096248825778No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG8690.12520242739988127No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA8660.12477019807629135No Hit
GCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA8570.12347351010552159No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8560.12332943366432496No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8500.1224649750171451No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT8450.1217445928111619No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC8450.1217445928111619No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT8230.11857491110483578No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8210.11828675822244249No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC8140.11727822313406602No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8020.11554930583970631No Hit
ATGCATAACATAGCTACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTC8010.11540522939850967No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG7940.11439669431013318No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT7750.111659241927397No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC7640.11007440107423394No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG7620.10978624819184067No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACCAGTCAGA7570.10906586598585745No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA7470.10762510157389105No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC7380.10632841360312126No Hit
ATGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAATTCCAAG7310.1053198785147448No Hit
GATAAAGAGTGTTCTTGGAATTGTCTCTGGAGATGGTGAATCGGCCCTCC7300.10517580207354814No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC7230.10416726698517166No Hit
CCTCCAGTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCCTCCA7200.10373503766158174No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG7120.1025824261320086No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG7060.10171796748482875No Hit
CTCTTTATCTGCAAATGAACAGCCTGAGAGGTGAGGACACGGCTGTATAT6980.10056535595525562No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)