FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254826
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA88970.7090226055245906No Hit
GTATATTACTGTGCGAAAACAATGACTACGGTGACTACGGCCTTTGACTA60370.4811025592392889No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT39400.3139877560713597No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCG32440.25852189865367786No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA31170.24840097352142845No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC30060.23955512557119474No Hit
GTATGTGCTACCACCACTACCACTAATAGCTGAGACCCACTCCAGCCCCT26960.21485050516964105No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26760.21325665869212146No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26160.20847511925956266No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC25580.20385296447475587No Hit
GCCGTATATTACTGTGCGAAAACAATGACTACGGTGACTACGGCCTTTGA25250.20122311778684854No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23640.18839265364281582No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA23340.1860018839265364No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG23070.18385019118188498No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT22130.1763591127375429No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA20820.16591941830978957No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20580.16400680253676606No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT20320.1619348021159906No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20250.16137695584885872No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA20110.160261263314595No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19370.15436403134777252No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGG19250.15340772346126075No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGG19250.15340772346126075No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG18930.15085756909722942No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT18560.14790895311381816No Hit
ATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT18520.14759018381831424No Hit
ATATTACTGTGCGAAAACAATGACTACGGTGACTACGGCCTTTGACTACT18350.1462354143124226No Hit
TCTATAGGGATAGCAACCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGC17870.14241018276637557No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCT17330.13810679727707267No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT17240.13738956636218885No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCC17070.1360347968562972No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT16780.1337237194638938No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC16500.13149233439536637No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT16240.1294203339745909No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA16170.12886248770745903No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC15860.12639202566730368No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC15840.1262326410195517No Hit
GCGTAGTATGTGCTACCACCACTACCACTAATAGCTGAGACCCACTCCAG15750.12551541010466788No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA15700.12511694848528798No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC15700.12511694848528798No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG15420.12288556341676056No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG15380.12256679412125666No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15130.12057448602435716No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG14980.11937910116621747No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG14430.11499602335303857No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC14250.11356156152327096No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14230.113402176875519No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14120.11252556131288322No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG14040.11188802272187538No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC13970.11133017645474352No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT13460.10726586793706856No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG13150.10479540589691319No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC13140.10471571357303722No Hit
TTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGC13100.1043969442775333No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC13010.10367971336264947No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT12840.10232494385675782No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA12800.10200617456125392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14200.053.2734681
ATCAACG14450.052.341343
TCAACGC14900.050.271114
TATCAAC16000.047.272662
CAACGCA16200.046.2370075
AACGCAG16950.044.1752666
GGCAGCG8700.041.3776937
CAGCGCT8600.040.184489
CGCAGAG18800.039.4452678
TATACGG36250.038.1473735
ATACGGC36500.037.6752436
GCAGCGC9800.036.733268
GTAATAT38400.036.398251
CTAACCG800.002102640536.0158273
TCGTGCG800.00210758535.9985969
ATACGCC1001.4497983E-435.9985926
AATATAC39400.035.8330043
TAATATA39950.035.7017172
ATATACG41050.034.037684
GTATGTA17100.033.705741
Produced by FastQC (version 0.11.5)