FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009772
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA14840.14696386907143397No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA13140.13012838541769825No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA13060.12933612736340483No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC10780.10675677281604165No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG10650.1054693534778148No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA10260.10160709546313425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18500.081.883141
ATCAACG24350.059.512293
TCAACGC25400.057.052144
CGCAGAG27250.053.403668
ACGCAGA27650.052.3705377
TATCAAC29850.049.5129852
GCAGAGT30850.047.636479
GGTATCA9750.041.431471
AACGCAG36900.039.2424246
CAACGCA37050.038.921945
GTACATG41900.035.465011
TACATGG40750.034.853642
ACATGGG40650.034.584673
CATGGGT8600.031.017934
GCCCATA8500.030.5513551
CATAACG1000.00624269528.8382932
TTACACG5250.028.8382914
GTTAGAC1555.714048E-527.9080263
CAGAGTA32300.026.7635239
TACACGC5200.026.345295
Produced by FastQC (version 0.11.5)