FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929603
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCAGCTCTCAGGCCGCTCATTTGTAGATACA18390.1978263839509984No Hit
GTTTATTACTGTGCGAGAGGCTTTGACGATAGTAGTGGTTATTTCTATGA17410.18728424929781853No Hit
ACTATACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC14300.15382910769435984No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCC11790.12682833424590928No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11060.11897551965731608No Hit
TTTAAGAGGTGTCCACTGCCAGGTGCAACTGGTGGAGTCTGGGGGAGGCG10550.11348930672555918No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10150.10918639462222045No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT9850.10595921054471641No Hit
CTACTATACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG9690.10423804570338091No Hit
CTCCATAAGGGGCCGATTCACCGTTTCCAGAGACAACTCCAAGAAGTCTC9690.10423804570338091No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9390.10101086162587684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10950.049.9250641
ATCAACG10450.048.8484083
GGTATCA5600.048.810661
TCAACGC10400.048.397164
TATCAAC11550.044.2033272
GAATACG2550.039.523789
CAACGCA13100.038.4221765
AACGCAG14350.036.0618936
GTACATG21100.035.79531
TACATGG21100.035.442922
CGCAGAG14450.035.372128
GCGTATG850.002849763733.850121
ACGTAGT900.003770675331.9695571
TTATACG3500.030.8161544
TATTGAG8850.028.436755
GTGCTAG3650.027.5901681
ATACGAT4300.026.7435766
CAGCGCT5950.026.6180579
TACGATG4100.026.337717
CTAGAGA5350.025.5361254
Produced by FastQC (version 0.11.5)