FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707214
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGACTGGTCCAAGAACCAGTTCTCCCTGAAGCTGACCTCTGTGAC32730.46280192417005317No Hit
CTACTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGGCT22680.3206950088657747No Hit
GTATAGTAGTTATTGGACGTTCGGCCAGGGGACCAGGGTGGAAGTCAAAC22290.3151804121524744No Hit
GTCTATGGATATGGTGACTCGACTCTCGAGGGACGGATTGTAGTGGGCGC19530.2761540354121949No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT18220.25763064645213474No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13930.19697008260583077No Hit
GAGTATTACTGAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC12950.18311289086471705No Hit
CTATAAGACGTCTCGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCA12880.18212309145463748No Hit
ACTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGGCTGC11660.16487230173610817No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTACTGA10070.14238971513572976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9880.13970311673694244No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9810.13871331732686287No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG9570.13531971934944725No Hit
GTCTTATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCA9270.13107772187767777No Hit
CAGTATAGTAGTTATTGGACGTTCGGCCAGGGGACCAGGGTGGAAGTCAA8970.12683572440590826No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8900.12584592499582872No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8460.11962432870390009No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8360.1182103295466436No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8230.11637213064221014No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG7850.11099893384463543No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7650.10817093553012244No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7440.10520153729988377No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA7320.10350473831117596No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7200.10180793932246816No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16900.066.519681
ATCAACG18150.061.9340483
TCAACGC18700.060.1124574
CAACGCA19050.059.3862885
AACGCAG19700.057.426846
TATCAAC21300.052.7747882
ACGCAGA22050.050.947287
CGCAGAG22300.050.688298
TAGACTG12750.046.9082685
TCCATAG13000.045.455111
CCATAGA13050.044.7255862
ATAGACT13400.044.6328664
CATAGAC13400.043.5573773
GCAGAGT26400.042.8162469
ACTGGTC14450.040.856298
GAATACG1601.9808922E-631.4985779
GACTGGT21000.030.5193827
GTCTATG11250.030.1063121
GTACATG21600.029.692621
TACATGG21800.028.7570462
Produced by FastQC (version 0.11.5)