FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154079
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT53620.46461290778187625No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47840.4145296812436584No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29260.2535355032021205No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24350.21099075539889384No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG22610.19591379792891125No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22180.19218788315184662No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22160.1920145847901227No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT21390.1853425978637511No Hit
GTGATGGATACACCAACTTCAATTGCTTTCAGCAGAGGCCAGGCCAATCT20700.17936380438427524No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA18380.1592611944242985No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17960.15562192882809583No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG17850.15466878783861418No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17670.15310910258309873No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17260.14955648616775802No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17100.14817009927396652No Hit
ACATTACCCCTCCAACATTCGGCCAGGGGACCAGGGTAGAAATCAAACGA16570.14357769268828216No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16000.13863868937914994No Hit
GAGTTACATTACCCCTCCAACATTCGGCCAGGGGACCAGGGTAGAAATCA15910.13785884675139223No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG15390.13335308934656986No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA14660.12702769914364614No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC14490.12555466306899266No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT14150.12260859091968573No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC13740.11905597450434502No Hit
GGGTAGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG13690.11862272860003518No Hit
CTATTGTACGAGAGGGTCGGGCATAGTCTCACCTGCAACTTACTACAAGT13570.11758293842969154No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13550.11740964006796761No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13240.11472351546124658No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12860.11143084658849177No Hit
ATACTACTGTCAACAGAGTTACATTACCCCTCCAACATTCGGCCAGGGGA12820.1110842498650439No Hit
ACATACTACTGTCAACAGAGTTACATTACCCCTCCAACATTCGGCCAGGG12790.11082430232245799No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12650.10961121379039042No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12570.10891802034349468No Hit
TTTAAAAGGTGTCCAATGTGAGGTGTACCTGGTGGAGTCTGGGGGAGGCT12450.10787823017315105No Hit
GTCGGAGACAGAGTTATCATCACTTGCCGGGCAAGTCAGAGCATTAGTAG12190.10562535147073987No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12150.105278754747292No Hit
GGGTACAACTGGCCTCCTATTCACTTTCGGCCCTGGGACCAAAGTGGATA12100.10484550884298216No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12020.1041523153960864No Hit
GCCGTGTACTATTGTACGAGAGGGTCGGGCATAGTCTCACCTGCAACTTA11990.1038923678535005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11950.10354577113005262No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11670.1011195940659175No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCGTGAGATTCCCAGGAGT11570.1002531022572978No Hit
GTCTGCATCTGTCGGAGACAGAGTTATCATCACTTGCCGGGCAAGTCAGA11550.10007980389557389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA22500.066.27875
TCAACGC22750.065.553214
ATCAACG22850.065.266333
AACGCAG23450.063.228846
ACGCAGA26500.055.9515577
CGCAGAG27150.054.6120158
TAACGCA450.00988214148.030134
GCAGAGT32350.046.056079
GTACATG30900.043.186291
TACATGG30350.042.728612
CATGGGG18650.036.312324
GTATCAA42600.036.0663871
TACGCTA800.002110487835.988269
TATCAAC43050.034.809292
ACATGGG42550.031.1546763
TATACGC950.004898153730.30597
GTATATA4500.027.2501581
ATACCGT1358.4329327E-426.657976
ATGGGGA13300.026.5418135
CAGAGTA33700.025.84329
Produced by FastQC (version 0.11.5)