FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008100
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG35670.3538339450451344No Hit
GTGTATTACTGTGCGAGAGATCTGCCCATACCAACTCGTCCAAGATACTT29430.29193532387659954No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTA26430.262176371391727No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA26170.2595972621763714No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23300.23112786429917664No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21770.2159507985318917No Hit
CCACTATAGTAGAGCAAGCAGTAATACTCAGCCTCATCCTCAGGCTGCGC21760.21585160202360876No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21720.21545481599047714No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21590.21416526138279932No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21350.21178454518400955No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT21250.21079258010118043No Hit
TTCCAGGACAGAGTCACCATTACCGCGGACGAATCCACGAGCACAGCCTA19910.19750024799127072No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19810.1965082829084416No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA19620.19462354925106637No Hit
CTATAGTGGTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAG19110.18956452732863804No Hit
CTACTATAGTGGTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC18420.18271996825711734No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT18370.1822239857157028No Hit
CTATAGTAGAGCAAGCAGTAATACTCAGCCTCATCCTCAGGCTGCGCACC16980.16843567106437854No Hit
CTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG16480.16347584565023313No Hit
ATAGTAGAGCAAGCAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCG16230.1609959329431604No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA16180.16049995040174586No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG16020.15891280626921933No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG15410.15286181926396192No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAG15390.1526634262473961No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15280.1515722646562841No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAGCACCACTA14780.14661243924213868No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14530.14413252653506597No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG14440.1432397579605198No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT14130.14016466620374962No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT14040.13927189762920344No Hit
GTCCTGGAACTTCTGTGCGTAGTTTGCTGTACCAAAGACAGCGATGATCC13870.137585556988394No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13810.13699037793869656No Hit
CTCTACTATAGTGGTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGT13710.13599841285586747No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT13240.13133617696657077No Hit
GCCTGAGGATGAGGCTGAGTATTACTGCTTGCTCTACTATAGTGGTGCTC13120.13014581886717586No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG12840.12736831663525444No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA12800.12697153060212282No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12730.12627715504414244No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG12620.12518599345303047No Hit
GTATTACTGTGCGAGAGATCTGCCCATACCAACTCGTCCAAGATACTTTG12590.12488840392818172No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCGCTGTCTTTGGT12510.12409483186191846No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12260.12161491915484576No Hit
CATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAATGGTGACTCTGTCCT12240.12141652613827994No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12230.12131732962999701No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12220.1212181331217141No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12200.1210197401051483No Hit
CCATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAATGGTGACTCTGTCC12170.12072215058029957No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA12120.12022616803888503No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA11980.11883741692292431No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11810.11715107628211487No Hit
GGGTACGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATC11780.11685348675726615No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA11710.11615911119928579No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG11540.11447277055847635No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11350.11258803690110109No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11100.11010812419402836No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11000.10911615911119929No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG10930.10842178355321892No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGG10900.1081241940283702No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCA10890.10802499752008729No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC10720.10633865687927785No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA10530.10445392322190258No Hit
GTATTACTGCTTGCTCTACTATAGTGGTGCTCGGGTGTTCGGCGGAGGGA10470.10385874417220514No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG10460.10375954766392223No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT10340.10256918956452732No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10310.1022716000396786No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10240.10157722448169826No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT10210.10127963495684952No Hit
ACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGT10160.10078365241543497No Hit
GCCCATACCAACTCGTCCAAGATACTTTGACTCCTGGGGCCAGGGAACCC10140.10058525939886916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6750.075.8635251
GTATCAA16850.068.913511
TCAACGC19050.059.416964
ATCAACG19100.059.2614253
TATCAAC20050.056.8131032
CAACGCA20350.055.6185265
AACGCAG21650.052.8765266
CGCAGAG23900.047.8867348
GCAGAGT27950.040.9478659
ACGCAGA29300.039.0611887
GAATACG1406.9923044E-735.9903459
GGCGTAA800.002109762335.990348
GTACATG26650.035.1823271
ACATGGG25950.034.7278943
CAGCGCT5000.034.5507249
ATACACG19100.033.5940554
AATACAC21150.032.042273
TAATACA22000.030.8042742
TATTGAG11000.030.8027445
GTATGTA13000.030.5139031
Produced by FastQC (version 0.11.5)