FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914791
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTGTGTGAGGGGGCAAGGGTCTTCCTCTCTTTGGGGGAGTCC41280.45125061352811735No Hit
GTAATATACAGCCGTGTCTTCAGGTCTCAGACTGGTCAATTGAAGATACA37890.41419296866716004No Hit
GTCTGAGACCTGAAGACACGGCTGTATATTACTGTGTGAGGGGGCAAGGG26670.2915420024901863No Hit
ATATTACTGTGTGAGGGGGCAAGGGTCTTCCTCTCTTTGGGGGAGTCCCG24580.2686952538885931No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20190.22070615036658647No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT19490.21305412930385192No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19340.2114144105046945No Hit
ATACTACGCAGACTCCGTGGAGGGCAGATTCACCATCTCCAGAGAGAATT19120.20900948959926366No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19110.20890017501265318No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18870.2062766249340013No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18860.20616731034739083No Hit
GCTGTATATTACTGTGTGAGGGGGCAAGGGTCTTCCTCTCTTTGGGGGAG18720.2046369061348439No Hit
ACCTATAGCAGTGCCCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGA17300.18911423483615383No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17200.1880210889700489No Hit
ATGCAATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGACTCGAATATGTT17040.186272055584281No Hit
CAGTAATATACAGCCGTGTCTTCAGGTCTCAGACTGGTCAATTGAAGATA15940.17424745105712672No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG15320.1674699466872761No Hit
ATATACAGCCGTGTCTTCAGGTCTCAGACTGGTCAATTGAAGATACAAGG15310.16736063210066562No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG13980.15282179208146998No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13870.15161933162875454No Hit
GCTATAGGTTAGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGC13720.14997961282959713No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13700.14976098365637616No Hit
GTCTCAGACTGGTCAATTGAAGATACAAGGTGCTCTTGGAATTCTCTCTG13560.14823057944382925No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12540.1370804916095589No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT12540.1370804916095589No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA12420.13576871657023298No Hit
CTTTGGGGGAGTCCCGGACTTGACTACTGGGGCCAGGGAACCCTGGTCAC11830.12931915596021387No Hit
CCCTGAGACTCTCCTGTTCAGGCTCTGGATTCAACTTCGCGGACTATGCA11140.12177644948408982No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG11020.12046467444476389No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10680.11674797850000712No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA10320.11281265338202934No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10190.11139156375609292No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10190.11139156375609292No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC10190.11139156375609292No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG10030.10964253037032504No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGGCCCGGGATTCCCAGGTGT9900.10822144074438861No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG9780.10690966570506269No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC9630.1052699469059053No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT9520.10406748645318985No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT9520.10406748645318985No Hit
CTCCTGTTCAGGCTCTGGATTCAACTTCGCGGACTATGCAATGCACTGGG9480.10363022810674788No Hit
GCGTAGTATGTGTCAAAACCCCGACTAGGAATACCGGAAACATATTCGAG9250.10111599261470652No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC9220.10078804885487505No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9220.10078804885487505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)