FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559654
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG26860.47993939112380146No Hit
GATTATTACTGTGCAGCATGGGATGACAACCTGAATGGCCCTAGTTGGGT18690.3339563373084084No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA16740.2991133807674027No Hit
ATAATACACGGCCGTGTCCTCGGATCTCAGGCTGCTCAGCTCCATGTAGG15060.2690948335936132No Hit
GTTCCTCACTATGGACTGGATTTGGACGATCCTCTTCTTGGTGGGAGCAG13020.23264374059686876No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12320.2201360126077898No Hit
CAATAATACACGGCCGTGTCCTCGGATCTCAGGCTGCTCAGCTCCATGTA12200.21799183066680486No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA11750.20995114838811124No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11400.20369728439357174No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCCGAGGACACGGCCGTGTATTA11160.1994089205116018No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC10720.1915469200613236No Hit
GTATTATTGTGCGGCAGATCCCTCTGGGTTTGGAATGGTAACCAAACGTT10700.19118955640449278No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT10190.18207678315530668No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9680.17296400990612057No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9470.16921169150939688No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9360.16724619139682734No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9310.16635278225475025No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA8840.1579547363192258No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTCGGCTGA8540.15259428146676338No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8530.15241559963834797No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC8430.15062878135419386No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8360.14937800855528593No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8310.14848459941320888No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8230.14705514478588558No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT8170.1459830538153931No Hit
GTGTATTATTGTGCGGCAGATCCCTCTGGGTTTGGAATGGTAACCAAACG7580.13544082593888368No Hit
CCATGTAGGCTGTGCTTGTGGACGTGTCCCTGGTAATGGTGACTCTTCCC7540.13472609862522203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.12954432560117501No Hit
CATGTAGGCTGTGCTTGTGGACGTGTCCCTGGTAATGGTGACTCTTCCCT7020.1254346435476205No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTT6920.12364782526346635No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6830.12203968880772763No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6810.1216823251508968No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC6690.11953814320991184No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG6680.11935946138149642No Hit
GTTATGCAGTGGGTGCGACAGGCTCGTGGACAACCCCCTGAGTGGATAGG6680.11935946138149642No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC6680.11935946138149642No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC6560.11721527944051147No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG6540.11685791578368063No Hit
GGATAGGATGGATCGTCGTTGGCAGTGGTAACACAAACTACGCACAGAAG6510.11632187029843438No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG6360.11364164287220317No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT6320.11292691555854152No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACTAGGGCCAT6220.1111400972743874No Hit
ATACACGGCCGTGTCCTCGGATCTCAGGCTGCTCAGCTCCATGTAGGCTG6130.10953196081864866No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA6110.10917459716181784No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6080.1086385516765716No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6070.10845986984815618No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG6070.10845986984815618No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA6060.10828118801974078No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6010.10738777887766371No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG5980.10685173339241745No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5920.10577964242192497No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC5840.10435018779460167No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA5810.10381414230935541No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT5810.10381414230935541No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC5790.10345677865252459No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT5780.10327809682410917No Hit
GTCCTGGGTCAGCCGAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC5730.10238468768203211No Hit
GTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATC5710.10202732402520129No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA5680.10149127853995504No Hit
ATCCTCTTCTTGGTGGGAGCAGCGACAGGTGCCCACCCCCAAATGCAGCT5660.10113391488312422No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA5620.10041918756946255No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)