FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559654
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAACCTGAATGGCCCTAGTTGGGT19050.34038888313136334No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG17360.3101916541291584No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA15810.28249597072476923No Hit
ATAATACACGGCCGTGTCCTCGGATCTCAGGCTGCTCAGCTCCATGTAGG14560.26016074217284246No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13870.24783169601217897No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC12280.21942128529412816No Hit
CAATAATACACGGCCGTGTCCTCGGATCTCAGGCTGCTCAGCTCCATGTA11900.21263137581434244No Hit
GTTCCTCACTATGGACTGGATTTGGACGATCCTCTTCTTGGTGGGAGCAG11860.21191664850068076No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT11690.20887905741761875No Hit
GTATTATTGTGCGGCAGATCCCTCTGGGTTTGGAATGGTAACCAAACGTT11090.1981581477126939No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10480.1872585561793537No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10430.18636514703727658No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCCGAGGACACGGCCGTGTATTA10280.1836849196110454No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9850.1760016009891826No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9350.16706750956841193No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8970.1602776000886262No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA8820.15759737266239499No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8780.15688264534873334No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8720.15581055437824085No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8610.1538450542656713No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8550.1527729632951788No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC8420.15045009952577842No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA7860.14044391713451526No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTCGGCTGA7820.13972918982085358No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT7800.13937182616402277No Hit
GTGTATTATTGTGCGGCAGATCCCTCTGGGTTTGGAATGGTAACCAAACG7680.13722764422303782No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7560.13508346228205284No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC7340.13115246205691375No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7280.13008037108642123No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC6990.12489859806237426No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6960.12436255257712801No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT6960.12436255257712801No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG6800.1215036433224814No Hit
CCATGTAGGCTGTGCTTGTGGACGTGTCCCTGGTAATGGTGACTCTTCCC6570.11739396126892689No Hit
GTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATC6460.11542846115635733No Hit
CATGTAGGCTGTGCTTGTGGACGTGTCCCTGGTAATGGTGACTCTTCCCT6410.11453505201428026No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6410.11453505201428026No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT6410.11453505201428026No Hit
GGATAGGATGGATCGTCGTTGGCAGTGGTAACACAAACTACGCACAGAAG6380.11399900652903402No Hit
GTTATGCAGTGGGTGCGACAGGCTCGTGGACAACCCCCTGAGTGGATAGG6380.11399900652903402No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6350.11346296104378777No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG6350.11346296104378777No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT6190.11060405178914114No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA6020.10756646070607913No Hit
ATACACGGCCGTGTCCTCGGATCTCAGGCTGCTCAGCTCCATGTAGGCTG6010.10738777887766371No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA5990.10703041522083287No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC5930.10595832425034038No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG5920.10577964242192497No Hit
GTCCTGGGTCAGCCGAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC5890.10524359693667873No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT5890.10524359693667873No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC5830.10417150596618627No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5820.10399282413777082No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG5810.10381414230935541No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC5780.10327809682410917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7100.045.6476631
TCAACGC7800.041.5436444
TATCAAC8150.038.876022
CAACGCA8400.038.5762445
GTATGTA6650.036.8232081
GTACACG5700.036.636155
AACGCAG8800.036.0012746
ACGGGGT5950.035.0936789
CGCAGAG9250.034.249868
TATGTAC6300.033.1469962
GGCAGCG3050.033.050357
GTACATG13850.032.760851
CACGGGG6900.032.3489728
CCTATAT900.003746308432.0097161
ACACGGG6550.031.8789887
TATTGAG8500.031.3450875
CATGGGG7000.030.8609944
GGTATCA4500.030.4092311
ACATGGG14450.030.3982563
CTCACTA8500.029.6507595
Produced by FastQC (version 0.11.5)