FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186229
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG14200.11970707173741327No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA12730.10731486078994865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25450.064.62121
ATCAACG29250.054.467483
TCAACGC29600.053.821164
GGTATCA12250.047.106611
CGCAGAG33500.046.8643078
ACGCAGA34900.044.9843677
GCAGAGT37200.042.203079
TATCAAC37900.042.0362472
CAACGCA39900.040.1098445
AACGCAG42550.037.23526
GTACATG48950.033.303031
GTATACG1301.7136852E-533.291691
TACATGG48450.031.9900972
ACATGGG52100.030.0256823
CATGGGG26000.028.8345034
AATCCGC6950.027.977546
TATACGG1400.001033253525.7461832
GCCCATA7800.024.0439991
AATGCGG2752.1007509E-723.5689567
GTATATA8950.023.3723771
Produced by FastQC (version 0.11.5)