FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013638
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGCGTGGTTGGGTGTTCGG27220.26853768307817977No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC19850.19582928027560134No Hit
GTCCTGGGCCCAGCCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC19490.19227771650234107No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG16780.16554233365363177No Hit
GTGCAGGGTCCTGGGCCCAGCCTGTGCTGACTCAGCCACCCTCAGCGTCT15970.15755131516379614No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGCCTGTGCTGACTCAGCCAC14290.14097735088858152No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT13840.13653789617200618No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13460.13278902330023146No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA13170.12992804137177177No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACGCAGG12680.1250939684581675No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC12680.1250939684581675No Hit
CCTCCAATATTGGAAGTAATTATGTGTTCTGGTACCAACAAGTCCCAGGA11860.11700429541907466No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11820.11660967722204574No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT11220.11069040426661195No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10840.10694153139483721No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA10380.10240342212900465No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10350.10210745848123295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16050.050.1789134
ATCAACG16300.048.9681433
CAACGCA17200.046.8216135
GTATCAA18750.044.5532071
AACGCAG18200.044.2511836
TATCAAC19500.041.6698652
CGCAGAG19550.040.8296788
GGTATCA7800.040.6237871
ACGCAGA19800.040.3121577
GAATACG4150.039.8546949
GTACATG30500.034.944931
TACATGG30400.033.115612
TAGCGCG900.003776830931.9591884
GCAGAGT25000.031.9288069
ACATGGG31150.031.8565923
GTGCTAG6800.030.712231
TATTGAG16150.030.275635
TTATTGA17900.030.1291264
GTATAGA4050.028.4503271
TCTACAC14000.028.2496413
Produced by FastQC (version 0.11.5)