FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725848
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCCGTG66090.3829421826255846No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT57560.33351720429609094No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40160.23269720160755755No Hit
ATAATACACAGCCGTGTCCGCGGCGGTCACAGAAGTCAACCTCAGGGAGA38460.22284697145982726No Hit
GTCCTGGAAGTATCCCTCAATGACAATTCCCCGAACGAAAGTGACAAGTC35570.2061015802086858No Hit
CCTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCACTTGACACGTCC34620.20059703983201302No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32800.19005149932091353No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31680.18356193592946773No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT31370.18176571749076398No Hit
GTGTATTATTGTGCGGGAGGACTTGTCACTTTCGTTCGGGGAATTGTCAT30790.17840505073447951No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC29140.16884453323815307No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTGAGTCCATTACCAC28460.16490444117906095No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27840.16131200430165346No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA27630.16009521116575734No Hit
GTATTATTGTGCGGGAGGACTTGTCACTTTCGTTCGGGGAATTGTCATTG27530.1595157858629497No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26690.15464861331936533No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG26370.1527944523503808No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC25790.14943378559409637No Hit
CACCATCAACAGTCTGCAAGCTGACGATTTTGCAACTTACTACTGTCAAC25630.1485067051096041No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25360.1469422567920234No Hit
GCATATATCAGGAGGTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAACT25310.14665254414061957No Hit
CAATAATACACAGCCGTGTCCGCGGCGGTCACAGAAGTCAACCTCAGGGA24980.14474044064135427No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACGGAACCTTG23830.13807704965906614No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG23180.13431078519081635No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22800.1321089690401472No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG22780.1319930839795857No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC22750.1318192563887434No Hit
GTAGTAAGTTGCAAAATCGTCAGCTTGCAGACTGTTGATGGTGAGAGTGA22650.13123983108593573No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22520.13048657819228576No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22500.13037069313172422No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT22350.12950155517751274No Hit
ACTTACTACTGTCAACAGAGTTTCACTGTCCCTCGGACGTTCGGCCAAGG22290.12915389999582813No Hit
TTCCAGGACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCCTCCAC21790.12625677348178982No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21600.12515586540645526No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21320.12353347455859381No Hit
GTCCATTACCACCTATTTGAGTTGGTATCAGCAGAAACCAGGAAAAGCCC21300.12341758949803228No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTT20840.12075223310511701No Hit
GGCTATGATGGGTCCTTCAGTGGTTTCTACTGGAGCTGGATCCGCCAGTC20780.12040457792343241No Hit
CCTGATATATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCC19780.11461032489535579No Hit
CCATCAACAGTCTGCAAGCTGACGATTTTGCAACTTACTACTGTCAACAG19270.11165525585103671No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18940.10974315235177143No Hit
CTTCCAGGACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCCTCCA18890.10945343970036758No Hit
GATTTCACTCTCACCATCAACAGTCTGCAAGCTGACGATTTTGCAACTTA18550.10748339367082155No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18520.10730956607997923No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA18450.10690396836801387No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT18350.1063245430652062No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA18330.10620865800464467No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC18280.10591894535324084No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC18230.10562923270183702No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG17950.10400684185397555No Hit
GGTTGTAGGTGGTGCCTCCACTATGATTGATTTCCCCAAGCCACTCCAGC17860.10348535908144867No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA17840.10336947402088711No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17830.10331153149060635No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA17470.10122560040049877No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA17370.10064617509769111No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT17350.10053029003712957No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)