FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917847
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16390.1785700666886747No Hit
GTATAAGTCTGTGTGCACAGAAATAAGTGGCTGTGTCCACAGGGTCCATG14110.15372932525791336No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT11200.12202469474759954No Hit
GACTTATACCACGTAGCCTCTTTGACTACTGGGGCCCGGGAACCCTGGTC10610.11559660814928849No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10380.1130907438821503No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20600.073.45471
TCAACGC22550.066.463674
ATCAACG23300.064.324283
CAACGCA23500.063.7768335
AACGCAG24450.061.0040936
TATCAAC24950.060.3591652
ACGCAGA27850.053.5448767
CGCAGAG29600.050.1085248
GCAGAGT43050.034.618619
GGTATCA16650.032.457461
GTACACG4400.031.1148995
ATACCGT1453.63967E-529.8160936
GTACATG24650.029.2314621
ATAAGTC9500.028.0637423
GTACGAT1306.719822E-427.7136786
TACATGG25400.027.517282
ACATGGG25500.026.8442273
CAGAGTA32900.024.9471769
GTATAAG13150.024.6577971
TAAGTCT11050.024.12724
Produced by FastQC (version 0.11.5)