FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917847
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGTCTGTGTGCACAGAAATAAGTGGCTGTGTCCACAGGGTCCATG13540.14751913990022303No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10730.11690401559301278No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10280.11200123767904673No Hit
GACTTATACCACGTAGCCTCTTTGACTACTGGGGCCCGGGAACCCTGGTC10060.10960432403221887No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15350.057.3268241
ATCAACG15000.056.7193343
TCAACGC14650.056.5979354
CAACGCA15700.052.812725
AACGCAG17400.047.600866
TATCAAC18150.047.2727362
ACGCAGA18950.043.7121627
CGCAGAG19900.041.263448
GTACATG27150.027.894971
TACATGG28100.026.4284232
GAATACG2508.338429E-825.9308139
GCAGAGT32350.025.6057329
CCCTATA3151.1023076E-925.1780742
ACATGGG29000.024.862363
TACACCG1550.001867390723.2583375
GTATAAG11700.022.8098431
CAGAGTA22350.022.5597449
ATAAGTC9050.022.3074443
TACCGTG2303.30337E-521.9221867
CGATAGA1700.003211463821.1853058
Produced by FastQC (version 0.11.5)