FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846233
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGGTCTCAGGCTATTCATTTGCAGATACA17150.2026628599924607No Hit
GTGTATTACTGTGCGAGAGACTCTTTCGTTTGGTACTATGCTTCGGGGAG11610.13719625682288447No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10340.12218856981469643No Hit
ATATTATATGATGGAAGTAGTGAATACTACGCAGACTCCGTGAAGGGCCG10330.12207039905085243No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10050.053.817121
ATCAACG10000.050.462523
TCAACGC10150.049.7167664
CAACGCA11350.044.4630055
AACGCAG12350.040.8192446
TATCAAC12850.040.3924562
CGCAGAG14450.035.8838048
GGTATCA5700.032.894541
GTACATG21000.029.5327851
GCAGAGT18750.027.2703639
TACATGG22600.026.4752792
ACATGGG23800.024.53463
CTTAGAC2101.6246042E-524.0297723
TATTCCG1550.001868364323.2559955
GAGTTAC11200.023.1482588
AGTTACC10900.023.1246629
CATGGGG15900.021.7628124
TCCGGAC1700.00319597521.202743
ACGCAGA24350.020.998747
GCACCGT2755.519614E-620.9503426
Produced by FastQC (version 0.11.5)