FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009036
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGATCA32070.31782810524104194No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30180.2990973562885764No Hit
GTTTTATACAGCTCCAGCAATATGAACTACTTAGCTTGGTACCAGCAGAA27620.27372660638470775No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24480.24260779595574392No Hit
TTATAGTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAAC24460.24240958697211995No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15120.14984599161972417No Hit
CTATAATATTGTTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCT15090.1495486781442882No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14720.1458818119472447No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14220.14092658735664537No Hit
TCTCTAGACACGTCCAAGAACCAGTTCTCCCTGCGGCTGACCTCTGTGAC13800.1367641987005419No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13790.1366650942087299No Hit
CTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTG13430.13309733250349837No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13260.13141255614269462No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12970.12853852588014697No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12650.1253671821421634No Hit
GTACTATAATATTGTTGACAATAATAAACTGCCACATCTTCAGCCTGCAG12580.12467345069947951No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12460.12348419679773567No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11820.11714150932176849No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11720.11615046440364862No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11250.11149255328848524No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11180.11079882184580135No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11030.10931225446862154No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11010.10911404548499756No Hit
TCCGAGGAGTACTATAATATTGTTGACAATAATAAACTGCCACATCTTCA10600.10505076132070611No Hit
GTCTCTGGTGGCTCCGTCAGTGGTGGTTCATTCTACTGGAGCTGGATCCG10450.1035641939435263No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10130.10039285020554273No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA657.594799E-444.303186
ACCGGTG1251.3274317E-534.556488
ACACCGG1402.8816692E-530.8539986
GTATCAA38750.027.570331
CATGGGG18850.026.3527534
TCAACGC41850.025.2877464
ATCAACG42100.025.137583
CAACGCA42700.024.7843595
TATCAAC43050.024.4350052
AACGCAG43150.024.3590476
ATTATAG23100.024.309213
CTAGTAC2101.6419788E-523.9975573
CCGGTGT1500.001556649123.9975559
CACCGGT1500.001556649123.9975557
GAGTTTA4251.8189894E-1223.778861
GTATAGA3753.255991E-1023.0994661
ACGCAGA45550.023.0755867
TTATAGT24700.023.0259934
ATATTAT26350.023.0118031
GTATAGT3803.8380676E-1022.7955251
Produced by FastQC (version 0.11.5)