FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009036
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGATCA29050.2878985487138219No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT26030.25796899218660185No Hit
TTATAGTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAAC20590.204056148640881No Hit
CTATAATATTGTTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCT20230.20048838693564947No Hit
GTTTTATACAGCTCCAGCAATATGAACTACTTAGCTTGGTACCAGCAGAA19310.19137077368894667No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17740.17581136847446474No Hit
GTACTATAATATTGTTGACAATAATAAACTGCCACATCTTCAGCCTGCAG16900.16748659116225784No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12370.12259225637142777No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12230.12120479348605998No Hit
TCTCTAGACACGTCCAAGAACCAGTTCTCCCTGCGGCTGACCTCTGTGAC12130.1202137485679401No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12130.1202137485679401No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11780.11674509135452056No Hit
TCCGAGGAGTACTATAATATTGTTGACAATAATAAACTGCCACATCTTCA11240.11139344879667326No Hit
CTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTG11190.11089792633761333No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10700.10604180623882598No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10470.10376240292715028No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAG900.003757262631.9931055
GTATTAG3650.027.6433641
GTACATG40800.026.6730421
ACAGTCG8300.025.1512099
TACACCG1751.318068E-424.6803975
GTCTTAT3001.8184437E-824.0234011
GTATCAA29450.023.7378921
CATGGGG19450.023.6864134
TCAACGC29150.023.4597844
ATCAACG29250.023.1334763
TATTATA26000.022.70282
CAACGCA30600.022.583375
CCTATAC3203.6516212E-822.4951533
ATTATAG22950.022.269713
TACATGG50150.022.2484462
ACATGGG46000.022.2212983
CTATACT4609.094947E-1221.9083214
GTCCTAT2653.979965E-621.7570421
AACGCAG31250.021.4225856
TATCAAC32000.021.3703962
Produced by FastQC (version 0.11.5)