FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679292
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21930.32283612938176803No Hit
GTATAATGACAGGCTCCCAATCACCTTCGGCCAAGGGACACGACTGGAGA21620.3182725543654275No Hit
TCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGTAGGCT14900.21934602497894867No Hit
GTAATACACGGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA14120.20786348138944669No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13740.20226942169199696No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC12740.18754821196186616No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTACAGTCTGAAGATT11620.1710604570641197No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11400.16782179092349092No Hit
GTCTCCAGCCACCCTGTCTGTGTCTTCAGGGGAAAGAGCCACCCTCTCCT11200.1648775489774648No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGGT10230.1505979755392379No Hit
GTGTATTACTGTGCGAGAGTCACAATTATTCCTGGGTTCTGGTTCGACTC10040.14780094569051305No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9670.14235409809036467No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9620.14161803760385813No Hit
ATGTAGAGTCTGTGAAGGGCCGATTCACCATCTCCAGGGACAACGCCAAG9420.13867379565783197No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGTAGGC9210.13558234161450453No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8970.1320492512792731No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA8830.1299882819170548No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGCCAGGTTCAGTG8600.12660240367912473No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT8590.12645519158182342No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8220.12100834398167504No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8170.12027228349516851No Hit
GTCTGTGTCTTCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA8140.11983064720326457No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAACAACTTAGCCTGGTACCAGC8070.11880016252215542No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8040.1183585262302515No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT7910.1164447689653345No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7800.11482543589502012No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7680.11305889072740441No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7240.10658155844614688No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7150.10525664957043511No Hit
TTATTACTGTCAGCAGTATAATGACAGGCTCCCAATCACCTTCGGCCAAG6950.10231240762440894No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC6950.10231240762440894No Hit
TATAAAAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGACTCT6890.1014291350406011No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14900.060.0455061
ATCAACG15750.055.3082353
TATCAAC16400.054.0298922
TCAACGC16150.053.9383744
CAACGCA16750.052.006255
AACGCAG17400.050.0634886
CGCAGAG19700.044.2185138
GGTATCA8000.041.4870871
ACGCAGA22150.039.3275267
GCAGAGT23100.037.710169
GAATACG1553.6890924E-837.1572239
GTACATG19600.033.4988941
ATACACG7200.032.9963874
TACATGG19700.032.545922
ACATGGG19700.032.158923
ACAGCGC1255.368501E-428.796858
GTACACG4050.028.4413325
GTAATAC8900.026.7527871
CCTATAC1658.82674E-526.1789553
CAGCGCT3358.185452E-1125.7882239
Produced by FastQC (version 0.11.5)