FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857325
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACACAATAATAAACGGCTGTGTCCTCGATTTCCAGACTATTCATT14120.16469833493715919No Hit
GTGTTAGACAAGGCGTTTGTATTGACACCGCCTGCGGTCCCTGGTTCTAC14050.16388184177528942No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11170.13028898025836178No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10900.1271396494911498No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10450.12189076487912984No Hit
CCATAGAACCAGAAGTTATGGTGGGACAACAAAATACGCCGCGTCTGTGA9980.1164085965065757No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9840.11477561018283615No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA9690.1130259819788295No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC9620.11220948881695972No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC9620.11220948881695972No Hit
GGATAGGACTCCATAGAACCAGAAGTTATGGTGGGACAACAAAATACGCC9550.11139299565508995No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC9230.10766045548654243No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6900.064.73321
GTATCAA18450.061.303851
TCAACGC17500.060.8983354
ATCAACG17850.059.711223
TATCAAC18300.059.4235082
CAACGCA18000.058.8066755
AACGCAG19050.055.5653576
CGCAGAG20800.050.881488
ACGCAGA20900.050.638037
GCAGAGT24650.042.934489
GTGCTAG3750.042.2645151
GAATACG2850.040.4185649
GTACATG21550.030.0870571
TACATGG21350.027.9970722
CTAGAGA4950.027.6394984
CAGAGTA23600.027.1508719
GTGTAGA4500.025.6148571
GTACTAA2701.7524144E-724.013931
GCTAGAG4800.024.0055243
TGCTAGA6350.023.8165042
Produced by FastQC (version 0.11.5)