FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857325
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACACAATAATAAACGGCTGTGTCCTCGATTTCCAGACTATTCATT14640.1707637127110489No Hit
GTGTTAGACAAGGCGTTTGTATTGACACCGCCTGCGGTCCCTGGTTCTAC13840.1614323622896801No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA11520.1343714460677106No Hit
CCATAGAACCAGAAGTTATGGTGGGACAACAAAATACGCCGCGTCTGTGA9680.11290934009856239No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9640.11244277257749394No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9380.10941008369054908No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA9220.10754381360627532No Hit
GTATTGACACCGCCTGCGGTCCCTGGTTCTACTACGGCCTGGACGTCTGG9210.10742717172600821No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG9160.10684396232467268No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA9050.10556090164173446No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC8960.10451112471933047No Hit
GGATAGGACTCCATAGAACCAGAAGTTATGGTGGGACAACAAAATACGCC8580.1000787332691803No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC10100.048.4928784
ATCAACG10350.046.6256453
TATCAAC10650.045.3201832
CAACGCA11000.044.522685
GTATCAA12450.042.2470131
TTAACGT700.001091192641.1578184
ACGCAGA11950.040.964097
AACGCAG12050.040.6241386
CGCAGAG12300.040.3837138
GTACATG20850.037.6671641
TACATGG21350.034.416662
GCAGAGT15750.031.994839
GGTATCA4850.029.7119241
ACATGGG25000.029.6747863
GAATACG3500.028.7953459
CATGGGG13400.027.4129494
GTATATA4200.025.7326471
GTATAGC2551.0070107E-725.429911
GGACTCG8650.024.9670626
GACTCGG8700.024.8235747
Produced by FastQC (version 0.11.5)