FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963795
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCACATACTATGCAGAATCCGTGAAGGGCCGGCTCACCTTGTCCAGAGA68650.3495782400912519No Hit
GTATGTGATATGACCAATTCCACTAATACTTGAGACCCACTCCAGCCCCT61140.31133595920144413No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC51130.260363225285735No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG46230.23541153735496834No Hit
GACCTAGGACGGTCAGCCTGGACCCTCCGCCGAACACCACTGGGTGATCA43960.22385228600744989No Hit
GTAATAGACGGCCTCATCCCCGGCCTCGACCCTGCTGATGGTCAGGGTGG39630.20180314136658867No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGACCCTCCGCCGAACACCAC36160.18413327256663756No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35960.18311483632456543No Hit
CTACTACCATCCCACACCTGACAGTAATAGACGGCCTCATCCCCGGCCTC32340.16468114034306025No Hit
CCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG31090.15831591383010957No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT30310.15434401248602833No Hit
GTCTATTACTGTCAGGTGTGGGATGGTAGTAGTGATCACCCAGTGGTGTT30030.1529182017471274No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29950.15251082725029852No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28970.1475204896641452No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA28850.1469094279189019No Hit
GGCTCTGTGATCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGC28310.14415965006530723No Hit
CACTACTACCATCCCACACCTGACAGTAATAGACGGCCTCATCCCCGGCC27890.1420209339569558No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26630.1356047856319015No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC26630.1356047856319015No Hit
GTAGTAGTGATCACCCAGTGGTGTTCGGCGGAGGGTCCAGGCTGACCGTC26550.13519741113507266No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA25670.13071629166995535No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC25590.1303089171731265No Hit
GTCCTGGTCTTCTGTTGTGATGGCGACCGGACCTCAGGGATCCCTGAACG25210.1283738883131895No Hit
TATCACATACTATGCAGAATCCGTGAAGGGCCGGCTCACCTTGTCCAGAG25140.12801743562846427No Hit
GCCTGGACCCTCCGCCGAACACCACTGGGTGATCACTACTACCATCCCAC24770.12613332858063087No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT24650.12552226683538759No Hit
ACCTAGGACGGTCAGCCTGGACCCTCCGCCGAACACCACTGGGTGATCAC23800.12119391280658114No Hit
GTACATGGGAGTCTGGGCCTGAGGAAGCAGCACTGGTGGTGCCTCAGCCA23700.12068469468554507No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA23440.11936072757085132No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23400.11915704032243692No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23330.11880058763771167No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23100.11762938595932876No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA23080.11752754233512154No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22960.11691648058987827No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC22250.11330103193052227No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT21990.11197706481582853No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21760.1108058631374456No Hit
TGGTAGTAGTGATCACCCAGTGGTGTTCGGCGGAGGGTCCAGGCTGACCG21640.11019480139220234No Hit
GTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACGGCCAG21460.10927820877433744No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21340.10866714702909418No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA20960.10673211816915718No Hit
CCTCTCTCACTGCACAGGCTCTGTGATCTCCTATGTGCTGACTCAGCCAC20740.10561183830287783No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20490.1043387930002877No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20370.10372773125504443No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19690.10026504803199926No Hit
GGATAACCTGTGGGGGAAACAACATTGGAAGAAAAAGTGTGCAATGGTAC19670.10016320440779204No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)