FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963795
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCACATACTATGCAGAATCCGTGAAGGGCCGGCTCACCTTGTCCAGAGA61200.3116414900740658No Hit
GTATGTGATATGACCAATTCCACTAATACTTGAGACCCACTCCAGCCCCT55490.282565135362907No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC46400.23627720816072958No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG40880.20816836787953935No Hit
GACCTAGGACGGTCAGCCTGGACCCTCCGCCGAACACCACTGGGTGATCA38630.19671096015622813No Hit
GTAATAGACGGCCTCATCCCCGGCCTCGACCCTGCTGATGGTCAGGGTGG35420.18036505847097076No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGACCCTCCGCCGAACACCAC33040.16824566719031264No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31380.15979264638111412No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30980.1577557738969699No Hit
CTACTACCATCCCACACCTGACAGTAATAGACGGCCTCATCCCCGGCCTC29840.15195068731715888No Hit
GTCTATTACTGTCAGGTGTGGGATGGTAGTAGTGATCACCCAGTGGTGTT28010.14263199570219906No Hit
CCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG27450.13978037422439715No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26220.13351699133565367No Hit
CACTACTACCATCCCACACCTGACAGTAATAGACGGCCTCATCCCCGGCC25910.13193841516044189No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25820.13148011885150945No Hit
GGCTCTGTGATCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGC25670.13071629166995535No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA24380.12414737790859025No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC24290.1236890815996578No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA24120.12282341079389653No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT24010.12226327086075685No Hit
GTAGTAGTGATCACCCAGTGGTGTTCGGCGGAGGGTCCAGGCTGACCGTC23640.12037916381292343No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC23570.1200227111281982No Hit
GTCCTGGTCTTCTGTTGTGATGGCGACCGGACCTCAGGGATCCCTGAACG23390.1191061185103333No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23180.1180367604561576No Hit
TATCACATACTATGCAGAATCCGTGAAGGGCCGGCTCACCTTGTCCAGAG22870.11645818428094581No Hit
GCCTGGACCCTCCGCCGAACACCACTGGGTGATCACTACTACCATCCCAC22220.11314826649421146No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA21870.11136600307058526No Hit
GTACATGGGAGTCTGGGCCTGAGGAAGCAGCACTGGTGGTGCCTCAGCCA21410.10902359971381942No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA21270.10831069434436894No Hit
ACCTAGGACGGTCAGCCTGGACCCTCCGCCGAACACCACTGGGTGATCAC20840.10612105642391391No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20510.10444063662449492No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20430.10403326212766607No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19660.10011228259568847No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28800.077.750291
TCAACGC35100.063.3896944
ATCAACG35500.062.472613
CAACGCA37950.058.4394655
TATCAAC39450.056.399962
GGTATCA14600.056.2193871
AACGCAG39400.056.1060146
CGCAGAG46200.047.6921358
ACGCAGA51250.042.9927147
GCAGAGT52400.042.1769189
GTACATG50700.040.7575451
TACATGG51050.039.9170072
ACATGGG52550.037.54443
CATGGGA30050.033.78644
ATGGGAG30500.030.6909855
GGGAGTC17750.028.396647
GTATGTG33200.027.9760071
CATACTA34600.027.8866275
ATATGAC30400.027.7127488
TATTGAG21200.027.511645
Produced by FastQC (version 0.11.5)