FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888079
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG32570.36674665204334295No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG31600.35582420032452067No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG28920.3256467048539601No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT25060.28218210316874964No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG24680.2779032045572522No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT22730.25594569852456817No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA22210.2500903635825191No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG20160.22700683159944104No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT18330.20640055670722987No Hit
GTATTACCACTGTCAGGGTTCATCCATCCCATCCACTCAAGCCCTTGTCC18310.20617535151715108No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC17260.1943520790380135No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT16850.1897353726413979No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15360.17295758598052652No Hit
GTGTATTACTGTGCGAGACAGGGCTCCGCGACTGTAGTACTAGCAGGACG14340.16147212128650718No Hit
CTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG14240.1603460953361131No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13860.15606719672461572Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12840.14458173203059638No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12770.14379351386532055Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12240.13782557632823206No Hit
GTATTTATGGAGGTGTTCCTGGTCATGGTGACTCTGCCCTGGAACTTCTG11590.1305064076506707No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC11110.12510148308877925No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC10930.12307463637806995No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT10450.11766971181617852No Hit
GTGGTAATACAGGCTATGCACAGAAGTTCCAGGGCAGAGTCACCATGACC10220.11507985213027219No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC10150.11429163396499636No Hit
GCTCCTCACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG9980.11237738984932646No Hit
CCATAAATACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACG9940.11192697946916885No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC9830.11068835092373538No Hit
ATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTG9790.11023794054357777No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG9640.10854890161798669No Hit
CTCCCAGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAGAGG9500.10697246528743501No Hit
GACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCA9440.10629684971719858No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9400.10584643933704097No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTA9340.10517082376680453No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC9150.10303137446105583No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT9030.10168014332058296No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA9030.10168014332058296No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)