FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888079
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG31350.35300913544853557No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG26330.2964826327387541No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25160.28330812911914366No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG25020.281731692788592No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA19820.22317834336810127No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT19140.2155213669054217No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17700.19930659321974734No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16830.1895101674513191No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC16390.18455565326958523No Hit
GTATTACCACTGTCAGGGTTCATCCATCCCATCCACTCAAGCCCTTGTCC16200.18241620396383657No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT12830.14446912943555698No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12790.14401871905539934No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12500.1407532437992566No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG12120.1364743451877592No Hit
GTATTTATGGAGGTGTTCCTGGTCATGGTGACTCTGCCCTGGAACTTCTG12000.13512311404728633No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT11360.1279165479647644No Hit
GTGTATTACTGTGCGAGACAGGGCTCCGCGACTGTAGTACTAGCAGGACG11140.12543929087389746No Hit
CTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG10980.123637649353267No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10520.11845792998145435No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA9860.1110261587088536No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC9160.10314397705609524No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC9080.10224315629578No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC9020.10156754072554357No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG803.8943195E-544.9917142
GAATACG2350.042.885729
GTCATAG12700.040.850481
TCATAGG13800.036.5150152
TAACGCA800.002108756235.9933744
CATAGGT13600.035.993373
ATAGGTG13100.035.1690944
TAGGTGG13450.034.789135
GTGCTAG4200.034.312241
CAGAGCG11550.033.0328759
CCGTTGA5100.032.4646079
CATTAGC4050.031.9941088
GTGGTCA14200.031.937788
ATGCAAC3250.031.0393811
CCCGTTG5350.030.947578
CCCCGTT5600.030.851467
AGGTGGT16050.030.0505396
ACCCCGT6050.029.7465886
TGCTAGA4600.029.7336522
CTAGTAG1607.1655726E-526.995033
Produced by FastQC (version 0.11.5)