FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836459
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCAGGTCTCAGGCTGTCCATTTGCAGAAATA26080.31179053605735607No Hit
GTCTATTACTGTTCGAGAGGGGAGTATAGCAGTAGTTGGTCCTCGGTTGA19870.2375490012062755No Hit
TTTAAGAGGTGTCCAGTCTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG16410.19618415248087473No Hit
AATAAATACTACGCAGACTCCGTGGAGGGCCGATTCTCCGTCTCCAGAGA15500.1853049581629225No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG14740.17621903763364374No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14740.17621903763364374No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14710.17586038287590905No Hit
GTATAGCAGTAGTTGGTCCTCGGTTGACACACCCTACTTTGACTACTGGG14660.17526262494635123No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14380.17191518054082747No Hit
GTCCAGGAGTGTCTGTTAGTTGTACTAAAAATCAGTGCCCTGGGTGCTTG13370.15984047036375962No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13200.15780809340326304No Hit
GTATTTATTACTTCCATCATATGTGATAACTGCCACCCACTCCAGCCCCT13100.1566125775441474No Hit
CTGCTATACTCCCCTCTCGAACAGTAATAGACAGCCGTGTCCTCAGGTCT12680.1515914109358618No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12360.14776576018669174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11610.13879939124332455No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11530.13784297855603203No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGATAGTGTCAGGGCAG11470.13712566904056264No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11460.1370061174546511No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC11020.1317458476745423No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10710.12803974851128389No Hit
GTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTA10370.12397499459029072No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10300.1231381334889098No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACCACCA10170.12158396287205947No Hit
ATACTACGCAGACTCCGTGGAGGGCCGATTCTCCGTCTCCAGAGACAATT10140.12122530811432479No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9200.10998745903863787No Hit
GTGGTGACTCAGGAGCCCTCATTGACTGTGTCCCCAGGAGGGACAGTCAC9170.10962880428090319No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTCAGTAACTATGCCATGTACTGGG8880.10616180828946786No Hit
CTCATTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCA8870.1060422567035563No Hit
GCTATACTCCCCTCTCGAACAGTAATAGACAGCCGTGTCCTCAGGTCTCA8520.1018579511966516No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT8380.1001842289938897No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)