FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836459
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCAGGTCTCAGGCTGTCCATTTGCAGAAATA24970.2985203100211726No Hit
GTCTATTACTGTTCGAGAGGGGAGTATAGCAGTAGTTGGTCCTCGGTTGA20260.24221151305682645No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG17280.2065851404551807No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16940.20252038653418755No Hit
TTTAAGAGGTGTCCAGTCTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG16060.19199984697397002No Hit
AATAAATACTACGCAGACTCCGTGGAGGGCCGATTCTCCGTCTCCAGAGA15150.18112065265601782No Hit
GTATAGCAGTAGTTGGTCCTCGGTTGACACACCCTACTTTGACTACTGGG14140.16904594247894997No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14100.1685677361353037No Hit
GTATTTATTACTTCCATCATATGTGATAACTGCCACCCACTCCAGCCCCT13570.16223150208199089No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13550.16199239891016773No Hit
GTCCAGGAGTGTCTGTTAGTTGTACTAAAAATCAGTGCCCTGGGTGCTTG12960.15493885534138555No Hit
CTGCTATACTCCCCTCTCGAACAGTAATAGACAGCCGTGTCCTCAGGTCT12620.1508741014203924No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12290.14692889908531082No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12200.14585293481210673No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11710.13999490710244017No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11560.13820163331376673No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGATAGTGTCAGGGCAG11540.1379625301419436No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC11490.1373647722123858No Hit
GTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTA10820.12935481595631107No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10160.12146441128614792No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10050.12014934384112072No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9420.11261759392869226No Hit
ATACTACGCAGACTCCGTGGAGGGCCGATTCTCCGTCTCCAGAGACAATT9270.11082432014001883No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9190.10986790745272633No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGCTCCTCCTCACTTGCT9160.10950925269499162No Hit
GTGGTGACTCAGGAGCCCTCATTGACTGTGTCCCCAGGAGGGACAGTCAC9010.10771597890631818No Hit
CTCATTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCA8650.10341212181350191No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACCACCA8620.10305346705576723No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8600.1028143638839441No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8560.10233615754029785No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTCAGTAACTATGCCATGTACTGGG8550.10221660595438628No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)