FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1635750
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA27570.16854653828519028No Hit
GTGTATTACTGTGCGAGGGCTCGGAACTGGAACTATCCCTACTACTACTA24760.15136787406388508No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22350.13663457129756992No Hit
GTCTCTGGTGGCCCCATCAGTATTACTAGTTACTACTGGGGCTGGATCCG21850.13357786947883232No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18800.11493198838453308No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16690.10203270670946049No Hit
ATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT16670.10191043863671098No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCA16450.10056548983646645No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33850.072.362221
TCAACGC38450.062.9286844
GGTATCA16350.062.1288031
CAACGCA39600.061.281185
ATCAACG39600.061.1012083
AACGCAG41950.057.6748476
TATCAAC43900.055.6050642
CGCAGAG49100.049.4198048
ACGCAGA51500.046.9769337
GCAGAGT60650.040.008459
GTATACG1157.4623385E-637.587641
GTACATG51850.031.1236741
TACATGG53600.029.0180442
ACATGGG57500.026.5505623
GTACACG10200.026.121255
GTACTAG3057.6579454E-1025.9827141
TACACGG10700.024.8998726
CCGATCG1851.9186919E-423.3524329
CAGAGTA55000.022.910159
GTATGTA15750.022.8707831
Produced by FastQC (version 0.11.5)