FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1635750
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA26230.16035457741097356No Hit
GTGTATTACTGTGCGAGGGCTCGGAACTGGAACTATCCCTACTACTACTA22870.139813541189057No Hit
GTCTCTGGTGGCCCCATCAGTATTACTAGTTACTACTGGGGCTGGATCCG22160.13547302460644964No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20550.12563044475011464No Hit
ATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT17760.1085740486015589No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCA17530.10716796576493964No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28900.073.9884951
GGTATCA13500.066.1292341
TCAACGC34200.060.633384
ATCAACG35650.058.167233
CAACGCA36650.056.5801285
AACGCAG37900.054.9040076
TATCAAC38300.054.14262
CGCAGAG45150.045.2903828
ACGCAGA47200.043.7809647
GCAGAGT53500.038.082469
GTACATG46400.029.946421
TACATGG48650.027.3800542
TACACGG8150.025.6203946
CAGAGTA48900.024.8811349
ACATGGG52500.024.0007153
GTATTAG9400.022.9772991
ACACGGG9850.021.9295857
GTATGTA13800.021.3899781
TATAACG2053.84252E-421.0737952
GTACACG10000.020.1605975
Produced by FastQC (version 0.11.5)