FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433075
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT55170.3849763620187359No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37900.26446627008356155No Hit
CCTTTGGCCAGGGGACCAAGCTGGAGCTGGATTTCAAACGAACTGTGGCT35910.2505800464037123No Hit
GGGTAAACACGTTCCGCACACCTTTGGCCAGGGGACCAAGCTGGAGCTGG32980.23013450098564275No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30180.21059609580796537No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27960.1951049317028069No Hit
TTATTACTGCATGCAGGGTAAACACGTTCCGCACACCTTTGGCCAGGGGA27150.18945275020497881No Hit
GGCCAAAGGTGTGCGGAACGTGTTTACCCTGCATGCAGTAATAAACCCCA27110.189173630131012No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26930.1879175897981613No Hit
CCCCTGGACAGCCGGCCTCCATGTCCTGCAAGTCTAGTCAGAACCTCCTG25710.1794044275421733No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT23780.16593688397327425No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG22710.15847042199466183No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21700.15142264012699963No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTGTCAGTGTGA21320.14877099942431485No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21070.14702649896202225No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21030.14674737888805542No Hit
GTATTGGTACCTGCTGAAGTCAGGCCAGTCTCCAAAACTCCTCTTTCATG20970.1463286987771052No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG19840.13844355668754252No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19120.13341939535613975No Hit
TGTTTACCCTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCG18770.1309770947089301No Hit
CCTGAATACTGATGGAAAGACGTATTTGTATTGGTACCTGCTGAAGTCAG18380.1282556739877536No Hit
CTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATGTCCTGCAAGTCTA18000.1256040332850688No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA17510.12218481237897527No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC17490.12204525234199186No Hit
GTTTATTACTGCATGCAGGGTAAACACGTTCCGCACACCTTTGGCCAGGG16840.11750955114003105No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16670.11632329082567207No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16550.1154859306037716No Hit
ATAATAAACACCCGTGTCGTCGGCTCTCAGACTGTTCATATGCAAGTAAA16540.1154161505852799No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTGTCAGTGT16430.11464857038187115No Hit
GTTCATATGCAAGTAAAGCGTGTTCTTGGTGTTGTCTCTGGAGACGGTGA16060.11206670969767807No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA15560.10857770877309282No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15170.10585628805191633No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA15130.10557716797794951No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA15030.10487936779303246No Hit
GTGTGAGGTGCAATTGGTGGAGTCCGGGGGAGGCTTATTTCAGCCTGGGG14980.10453046770057393No Hit
GACTTCACACTGACAATCAGCCGGGTGGAGGCTGAGGATGTTGGGGTTTA14660.10229750710883938No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14660.10229750710883938No Hit
GTGCAGCCACAGTTCGTTTGAAATCCAGCTCCAGCTTGGTCCCCTGGCCA14590.10180904697939745No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)