FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909553
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA23170.2547405153960242No Hit
GTTCCAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTC21620.23769917750807262No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21100.2319820835069534No Hit
GTCTCTGGTGACTCCATCAGCAAAAGTGATTACTACTGGGGCTGGATCCG18700.20559549580948006No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC17680.19438119603805384No Hit
CTCCTGCACTGGGACCAGCACCAACATCGGGGCAGGTTATGATGTACACT14660.16117807318539987No Hit
GATGTACACTGGTACCGGCAGCTTCCAGGCACAGCCCCCCAACTCCTCAT13300.14622567349016494No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13020.14314723825879305No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12210.1342417649108958No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11820.12995394441005637No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCAGTAGACACGTCC11230.12346724160109417No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCGCTCAGG10900.11983908579269159No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10770.11840981229241176No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10430.11467171236860305No Hit
GGTTATGATGTACACTGGTACCGGCAGCTTCCAGGCACAGCCCCCCAACT10170.11181316536804344No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10100.11104355656020046No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10080.11082366832938816No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9480.10422702140501983No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGA9370.1030176361355523No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9170.10081875382742951No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGAG8250.039.2634165
TCAACGC22150.032.172964
ATCAACG22500.031.0326423
TATCAAC23900.030.118382
CTATACC2901.2732926E-1129.786044
TTATTGA10950.029.5820264
GTATCAA25200.029.4620861
GTAGACG7600.028.4143165
ATTGAGT7150.028.189126
CAACGCA25100.028.104895
AACGCAG25750.027.6749926
CGCAGAG26300.027.3699368
TAGACGG7900.027.3352896
ACGCAGA26850.026.5411937
GTGCTAG2851.0521035E-825.2919251
TTAGGAC2651.4746365E-724.4470333
GGTTTAT8350.024.1712071
GTACATG33500.024.0990541
ACTATAC2401.7005023E-623.994313
TGACTCC14400.023.994319
Produced by FastQC (version 0.11.5)