FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927280
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGACTGGTCCAAGAACCAGTTCTCCCTGAAGCTGACCTCTGTGAC41500.44754550944698474No Hit
GTATAGTAGTTATTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAAC39470.42565352428608405No Hit
CTACTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGGCT36820.39707531705633675No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30710.33118367699076867No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25180.27154688982831504No Hit
GAGTATTACTGAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC23500.25342938486756966No Hit
GTCTATGGATATGGTGACTCGACTCTTGAGGGACGGATTGTAGTGGGCGC22610.24383142093003193No Hit
ACTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGGCTGC19100.20597877663704597No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16940.1826848416875162No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16660.17966525752739193No Hit
CAGTATAGTAGTTATTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAA16040.17297903545854543No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16020.1727633508756794No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG15880.1712535587956173No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTACTGA15880.1712535587956173No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15440.16650849797256492No Hit
GAATTCACACTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCGACTTA14960.1613320679837805No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14810.1597144336122854No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14610.15755758778362522No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13850.1493615736347166No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA13830.14914588905185058No Hit
GTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA13150.141812613234406No Hit
GTATTACTGAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT12740.13739107928565267No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12730.13728323699421965No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12260.13221464929686824No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12040.12984211888534206No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11700.1261754809766198No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT11140.12013631265637133No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG11120.1199206280735053No Hit
TCCAATAACTACTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGC10730.11571477870761797No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA10490.11312656371322577No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10340.11150892934173066No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA10330.11140108705029765No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10300.11107756017599862No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC10140.10935208351307048No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10050.1083815028901734No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9860.10633249935294625No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9720.10482270727288413No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9630.10385212664998705No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9520.10266586144422396No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCGACTTATTACTGCCAAC9500.10245017686135793No Hit
GTGTGAATTCTGTCCCATATCCACTGCCGCTGAACCTTGATGGGACCCCA9460.10201880769562591No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9450.10191096540419292No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17800.063.9557151
TCAACGC19450.058.5270084
ATCAACG19400.057.935093
CAACGCA20200.056.7106445
AACGCAG21200.054.0297856
TATCAAC21100.053.608792
CGCAGAG24550.046.6294068
ATAGACT14950.044.818844
TAGACTG15700.042.218915
TCCATAG15650.041.8956871
CATAGAC16250.041.67673
CCATAGA15750.041.6274342
GCAGAGT28750.039.817469
ACGCAGA28950.039.5637177
ACTGGTC17300.039.5359158
GAATACG2302.910383E-1134.433349
TTACACG1905.7680154E-934.1277544
CTGGTCC19450.034.055139
TCTATGG12850.032.5194852
GTCTATG12850.031.3998181
Produced by FastQC (version 0.11.5)