FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927280
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGTTATTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAAC39380.42468294366318693No Hit
TCCATAGACTGGTCCAAGAACCAGTTCTCCCTGAAGCTGACCTCTGTGAC39260.4233888361659909No Hit
CTACTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGGCT38130.4112026572340609No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29880.322232766801829No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22860.24652747821585713No Hit
GTCTATGGATATGGTGACTCGACTCTTGAGGGACGGATTGTAGTGGGCGC22620.24393926322146495No Hit
GAGTATTACTGAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC22270.24016478302130964No Hit
ACTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGGCTGC19540.21072383746009835No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16070.17330256233284444No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15560.167802605469761No Hit
CAGTATAGTAGTTATTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAA15500.16715555172116298No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15270.16467517901820378No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14480.15615563799499613No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG14370.154969372789233No Hit
GAATTCACACTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCGACTTA13820.14903804676041757No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTACTGA13810.14893020446898456No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13730.1480674661375205No Hit
GTGTGAATTCTGTCCCATATCCACTGCCGCTGAACCTTGATGGGACCCCA13170.14202829781727203No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12840.13846950219998275No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA12510.13491070658269347No Hit
GTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA12300.1326460184626003No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12180.1313519109654042No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11890.12822448451384694No Hit
GTATTACTGAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT11570.12477353118799069No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11000.11862652057630921No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10880.1173324130791131No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT10510.1133422482960918No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10270.11075403330169958No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10240.11043050642740057No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA10160.1095677680959365No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC10040.10827366059874041No Hit
TCCAATAACTACTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGC9890.10665602622724527No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9520.10266586144422396No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCGACTTATTACTGCCAAC9520.10266586144422396No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG9320.1005090156155638No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA9290.10018548874126476No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)