FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1151046
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACTCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA45200.39268630445698954No Hit
GTTTTATACAGCCCCAACAATAAGAATTACTTAGCTTGGTACCAGCAGAA32340.28096183818891685No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT31570.27427227061299025No Hit
GTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT29440.25576736290295954No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25750.22370956503910355No Hit
ATTATACTCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA18650.1620265393390012No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16340.14195783661122144No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16010.13909087907868148No Hit
GCTCATTAAGTGGGCATCTACCCGGGAATCCGGCGTCCCTGACCGATTCA15150.13161941399388036No Hit
TTATTACTGTCAGCAGTATTATACTCTCCCGCTCACTTTCGGCGGAGGGA14670.1274492939465495No Hit
ATAATACACAGCCGTGTCTTCAGATCTCAGGCTGCTCAACTCCATGTAGG14430.12536423392288404No Hit
CTCCTACGCTGCTCATTAAGTGGGCATCTACCCGGGAATCCGGCGTCCCT14280.12406107140809317No Hit
GTATTATTGTGCGACCCCCGCCCCCGAGTTTTACTATGGTTCGGGGGGAT13830.12015158386372048No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13630.11841403384399928No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13460.11693711632723626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13210.11476517880258479No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12800.11120320126215633No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12300.10685932621285335No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11940.10373173617735522No Hit
GTTTATTACTGTCAGCAGTATTATACTCTCCCGCTCACTTTCGGCGGAGG11690.10155979865270372No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11570.100517268640871No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11520.1000828811359407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23450.075.364081
GGTATCA8700.066.330291
CAACGCA28550.060.0596735
TATCAAC30100.057.4481122
AACGCAG30450.056.7335136
CGCAGAG36100.048.0535628
ACGCAGA36100.047.6547787
ATCAACG37850.045.494933
TCAACGC38050.045.25584
GCAGAGT40000.043.368349
GTACATG32550.032.5767331
ACATGGG33700.031.0005283
TATACTC28350.029.9875555
ATTATAC29750.028.8198013
TATACTG5000.028.8185485
GTACACG7050.028.6141625
TTATACT31250.028.3586864
GTATAAT19350.027.2134171
TATTATA32600.026.9633122
GTATTAT40000.026.8699871
Produced by FastQC (version 0.11.5)