FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1151046
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACTCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA41880.36384297412961775No Hit
GTTTTATACAGCCCCAACAATAAGAATTACTTAGCTTGGTACCAGCAGAA32520.2825256332066659No Hit
GTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT29270.25429044538619655No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27160.23595929267813798No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22610.19643002972948084No Hit
CTCCTACGCTGCTCATTAAGTGGGCATCTACCCGGGAATCCGGCGTCCCT16470.14308724412404022No Hit
ATTATACTCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA16130.1401334090905142No Hit
TTATTACTGTCAGCAGTATTATACTCTCCCGCTCACTTTCGGCGGAGGGA14490.1258854989288004No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14320.1244085814120374No Hit
ATAATACACAGCCGTGTCTTCAGATCTCAGGCTGCTCAACTCCATGTAGG14150.12293166389527438No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14040.12197601138442773No Hit
GCTCATTAAGTGGGCATCTACCCGGGAATCCGGCGTCCCTGACCGATTCA13220.11485205630357084No Hit
GTATTATTGTGCGACCCCCGCCCCCGAGTTTTACTATGGTTCGGGGGGAT12220.10616430620496488No Hit
GTTTATTACTGTCAGCAGTATTATACTCTCCCGCTCACTTTCGGCGGAGG11690.10155979865270372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15950.061.795131
CAACGCA17900.054.639935
AACGCAG18900.052.5282336
TATCAAC20200.048.393282
CGCAGAG20900.046.8172538
ACGCAGA23300.042.610597
GCAGAGT27550.035.5165379
TCAACGC27850.035.1110234
ATCAACG28250.034.856263
GTACATG32150.034.6852721
GGTATCA7500.033.5738531
ACATGGG33350.032.543163
CAGCGCT5350.029.5857269
CATGGGG23800.027.7933334
GTACACG8300.027.726575
GTATAAT20800.027.6707571
TACACGG8700.026.4609976
ATTATAC28900.026.11383
TTATACT29550.026.0349084
TATAATA22150.025.9605432
Produced by FastQC (version 0.11.5)