FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122727
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCGTCAACTGTCAGGCTGTTCATTTGCAGATCCA40210.35814583598684274No Hit
GTATGTGGGGAGAGGCATGTCGATGTTCGGCGGAGGGACCAAGCTGACCG37490.3339191094540347No Hit
CCCCACATACAGCGCACAGTAATAATCAGATTCATCATCTGCCTGGGCCC30680.2732632242744674No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG30440.2711255719333373No Hit
GATTATTACTGTGCGCTGTATGTGGGGAGAGGCATGTCGATGTTCGGCGG30140.2684535065069246No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC24780.22071260422168523No Hit
GTGTATTACTGTGCGAAAGGAGACCAGGGGAGTGGTTACTGGAGACTACT22130.1971093596217068No Hit
GTACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATC20670.18410530787983187No Hit
TATAGATACTATGCAGACTCCGTGAGGGGCCGATTCACCATCTCCAGAGA20600.18348182594700224No Hit
TTTAAGAGGTGTCCAGTGTCAGGTACACCTGGTGGAGTCTGGGGGAGGCG19050.16967615457720353No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT17040.15177331622023876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15990.14242108722779448No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15570.13868019563081674No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC15510.13814578254553422No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATCGACATGCCTCTC14800.13182189436969094No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14790.13173282552214385No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13630.12140083920668159No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13520.12042108188366361No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13290.11837249839008059No Hit
CTCGTCTACAGTACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTC12940.11525508872593249No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12890.11480974448819706No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA12600.11222674790933149No Hit
GTATCTATAAGTTCCGTCATATGACACAATCGCCACCCACTCCAGCCCCT12550.11178140367159603No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATCGA12470.11106885289121932No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12430.11071257750103096No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12410.11053443980593679No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12150.1082186497697125No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA12090.10768423668442997No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11980.10670447936141197No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT11590.10323079430707555No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11420.10171662389877503No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT11330.10091500427085123No Hit
GTCACATCCTGGCTGGAATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11240.10011338464292745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)