FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122727
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTGGGGAGAGGCATGTCGATGTTCGGCGGAGGGACCAAGCTGACCG38210.34033206647742503No Hit
GTAATACACAGCCGTGTCGTCAACTGTCAGGCTGTTCATTTGCAGATCCA38070.33908510261176583No Hit
GATTATTACTGTGCGCTGTATGTGGGGAGAGGCATGTCGATGTTCGGCGG30610.2726397423416378No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG27290.24306888495600446No Hit
CCCCACATACAGCGCACAGTAATAATCAGATTCATCATCTGCCTGGGCCC25070.2232956008005508No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC23230.20690693285188652No Hit
GTGTATTACTGTGCGAAAGGAGACCAGGGGAGTGGTTACTGGAGACTACT23070.2054818312911331No Hit
GTACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATC19970.1778704885515357No Hit
TTTAAGAGGTGTCCAGTGTCAGGTACACCTGGTGGAGTCTGGGGGAGGCG18490.1646882991145666No Hit
TATAGATACTATGCAGACTCCGTGAGGGGCCGATTCACCATCTCCAGAGA17410.15506886357948105No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA15000.1336032713206327No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT14770.13155468782704968No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC14190.12638869466931854No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13780.12273687191988793No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATCGACATGCCTCTC13510.12033201303611654No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13440.1197085311032869No Hit
CTCGTCTACAGTACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTC12540.11169233482404896No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA12390.11035630211084262No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATCGA12360.11008909556820136No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12260.10919840709273046No Hit
GTATCTATAAGTTCCGTCATATGACACAATCGCCACCCACTCCAGCCCCT12060.10741703014178869No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12050.10732796129424162No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12040.10723889244669452No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11750.10465589586782895No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11590.10323079430707555No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11470.10216196813651049No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11420.10171662389877503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8850.052.0500531
TCAACGC16900.051.907644
ATCAACG17000.051.1747633
GTATCAA18650.048.2409321
CAACGCA18300.047.157035
TATCAAC19400.044.8438642
AACGCAG19600.044.3981556
GAATACG4550.041.0976459
ACGCAGA21550.040.3806887
CGCAGAG22350.038.615248
ACCGACC3750.038.3578038
CAGCCGT17450.038.3303269
ACAGCCG17850.038.2772188
GTAATAC18750.037.2353061
GTACATG31800.034.4033661
TACATGG31950.032.8549962
AATACAC21100.032.7120823
ACATGGG31700.032.4336783
GCAGAGT27050.032.1716359
GCACCGA4300.031.7775766
Produced by FastQC (version 0.11.5)