FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935766
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCGTCGGACCGGAGCCCACTGATGGCCAGGGAGG65480.6997475864692668No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGG44920.48003453854916717No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC37510.40084807526668No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGG27580.29473180260877185No Hit
TTCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAATTGTTGGTACCA26760.2859689281294683No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22340.23873489739956355No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT22120.23638388229536017No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA21690.2317887164098717No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGAGACCC21660.2314681234411167No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20970.22409448515975147No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT20900.2233464348993231No Hit
GTAGTAAACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA20200.2158659322950396No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC19820.21180508802414277No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA18430.196950947138494No Hit
CTATAGGAATGATCAGCGACCCTCAGGGGTCCCTGACCGATTCTCTGGCT18330.19588230390931066No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA17260.18444782135704868No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16730.17878401224237683No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC16310.1742957106798067No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15910.17002113776307326No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15810.16895249453388989No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGC15410.16467792161715644No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15410.16467792161715644No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA15380.1643573286484014No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15270.16318182109629972No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15240.16286122812754472No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15050.16083080599209632No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14720.15730428333579122No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT14720.15730428333579122No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG14610.15612877578368953No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCG14440.15431208229407778No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14100.15067869531485437No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT13640.14576293646061086No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCGTCGGACCGGAGCCCAC13510.14437370026267252No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA13360.14277073541889745No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGAGACCCCCGGTCAGAGGGTCTC12910.13796184088757232No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12870.13753438359589898No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT12800.13678633333547063No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG12750.13625201172087895No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12660.13529023281461391No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12170.13005388099161544No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA11980.12802345885616703No Hit
CAGTAATAATCAGCCTCATCGTCGGACCGGAGCCCACTGATGGCCAGGGA11810.1262067653665553No Hit
GCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCT11460.12246651406441354No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11230.12000863463729179No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC11160.11926058437686345No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG10700.11434482552261999No Hit
GTTTACTACTGTGCGAAAGACATGACTCGTTTTGGAGTGGTTGTTCTGAA10500.11220753906425324No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG10400.11113889583506988No Hit
TTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAG10400.11113889583506988No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC10140.10836042343919314No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10100.1079329661475198No Hit
GCACAGTAATAATCAGCCTCATCGTCGGACCGGAGCCCACTGATGGCCAG9750.10419271484537801No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC9640.1030172072932763No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9620.10280347864743963No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9560.10216229270992962No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG9370.10013187057448124No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19700.071.71841
GGTATCA7250.063.6330531
ATCAACG22500.061.8290143
TCAACGC22700.061.2842644
TATCAAC23350.059.8869742
CAACGCA23500.059.1979945
AACGCAG24800.056.385526
CGCAGAG28450.049.0937468
TAGACGG803.8646736E-545.0502854
GCAGAGT32450.043.2639929
ACGCAGA33900.041.4378247
CTACGCA700.001087207241.1888354
TAAACGG8450.034.973955
GTAAACG8600.033.5257954
GTACATG24400.032.4970251
TAATACG900.00373269332.035764
AAACGGC9250.031.169936
TACATGG24700.030.9333132
CAGAGTA31150.030.7397489
GACGGGT950.004863240330.3496676
Produced by FastQC (version 0.11.5)