FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935766
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGG45440.4855914833409207No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC36610.3912302862040296No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGAGACCC29190.311936958598624No Hit
GTAATAATCAGCCTCATCGTCGGACCGGAGCCCACTGATGGCCAGGGAGG27980.2990063755255053No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT27530.29419748099418014No Hit
TTCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAATTGTTGGTACCA25800.27570995312930796No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23080.24664285729552046No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC21060.22505626406601648No Hit
CTATAGGAATGATCAGCGACCCTCAGGGGTCCCTGACCGATTCTCTGGCT20840.2227052489618131No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20830.22259838463889475No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGG20010.2138355101595912No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT20000.21372864583667286No Hit
GTAGTAAACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA19650.20998839453453105No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA18200.19449306771137229No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGAGACCCCCGGTCAGAGGGTCTC17910.19139400234674053No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC17640.18850866562794544No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16680.17824969062778515No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA16660.1780359619819485No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT16370.1749368966173167No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15410.16467792161715644No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15380.1643573286484014No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15260.16307495677338138No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA14780.15794546927330122No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14600.15602191146077118No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14430.15420521797115944No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14420.15409835364824112No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13970.149289459116916No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13730.1467247153668759No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT13570.14501488620018252No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCG13440.14362565000224414No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT13260.1417020921897141No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13200.14106090625220408No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG13200.14106090625220408No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA13000.13892361979383736No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA12100.12930583073118707No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12080.1290921020853504No Hit
GTCAGAGGGTCTCCATCTCTTGTTCTGGAAGCAGCTCCAATATCGGAAGA12000.12823718750200372No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11980.12802345885616703No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG11360.12139787083523018No Hit
GCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCT11330.12107727786647518No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG11130.11893999140810843No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC11020.11776448385600674No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGC11010.1176576195330884No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10820.11562719739764002No Hit
GTTTACTACTGTGCGAAAGACATGACTCGTTTTGGAGTGGTTGTTCTGAA10720.11455855416845666No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC10630.11359677526219161No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC10540.11263499635592658No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA10290.10996338828296817No Hit
TTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAG9860.1053682223974797No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9740.10408585052245967No Hit
TCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCA9640.1030172072932763No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG9550.10205542838701129No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG9460.10109364948074626No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG12750.063.327673
TCAACGC12650.063.2583924
GTATCAA14500.057.2038081
CAACGCA14450.055.3873525
TATCAAC14500.055.1874922
AACGCAG14800.054.509216
CGCAGAG15350.052.5589268
GGTATCA4550.052.311561
GCAGAGT18850.042.7999739
GTACATG25100.040.2299421
TAAACGG7850.039.4961055
TACATGG25400.039.167972
GTAAACG8400.036.0458834
ACGCAGA22500.035.856877
ACATGGG29650.033.5536733
AAACGGC9000.032.813686
ATGGGAG14650.032.4834255
TATTGAG16050.032.345435
AATAGCG950.004855687730.3593085
TTATTGA17400.029.4167524
Produced by FastQC (version 0.11.5)