FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225902
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG73940.6031477230643232No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGCGTGTTTGGGTGTTCGG49640.4049263317948743No Hit
GTAATACACGGCCGTGTCCTCGGATGTCAGGCTGCTCAGCTCCATGTAGG29070.23713151622234077No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27090.22098014360038568No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT24230.19765038314645053No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23700.19332703592946254No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC23080.18826953541147662No Hit
GACTTAGGACGGTAAGTATGGTCCCTGCGCCGAACACCCAAACACGCAGG22150.1806832846344977No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22120.18043856686749837No Hit
CTATTACTACTACGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCG21920.1788071150875029No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA21470.17513634858251312No Hit
GTATGGTCCCTGCGCCGAACACCCAAACACGCAGGCTGTCATCCCATGCT20730.1690999769965299No Hit
CCTCTATTACTACTACGCTATGGACGTCTGGGGCCAAGGGACCACGGTCA19520.15922969372755733No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCCTCTTCTTGGTGGGAGCAG19350.1578429597145612No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19300.15743509676956233No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG19210.15670094346856436No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19140.15612993534556596No Hit
GTAGTAATAGAGGCGTGTAACCACTCCAAACCCCCTCTGGTCTGCCGCAC18950.15458005615457027No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG18360.14976727340358365No Hit
TAATAGAGGCGTGTAACCACTCCAAACCCCCTCTGGTCTGCCGCACAGTA18000.14683066019959182No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA17920.14617807948759362No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA17810.14528078100859612No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT17790.1451176358305966No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA17520.1429151759276027No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17430.14218102262660473No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC17370.1416915870926061No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT17260.1407942886136086No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG17000.1386734012996145No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16930.13810239317661607No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGCGTGTTTGGGTGTTCGGCG16920.1380208205876163No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA16820.13720509469761857No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16520.13475791702762538No Hit
GCCTTGGGCTGACTTAGGACGGTAAGTATGGTCCCTGCGCCGAACACCCA15570.12700852107264693No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA15370.12537706929265147No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC15290.12472448858065327No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC15140.12350089974565667No Hit
GTGTATTACTGTGCGGCAGACCAGAGGGGGTTTGGAGTGGTTACACGCCT14800.1207274317196644No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT14570.1188512621726696No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAC14550.11868811699467005No Hit
CAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGA14370.11721981039267412No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT13990.11412005201068273No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13560.1106124306836925No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT13310.10857311595869816No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12870.10498392204270815No Hit
GCCTACATGGAGCTGAGCAGCCTGACATCCGAGGACACGGCCGTGTATTA12840.10473920427570883No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12830.10465763168670905No Hit
TTACTGTGCAGCATGGGATGACAGCCTGCGTGTTTGGGTGTTCGGCGCAG12580.10261831696171471No Hit
GCATGGGATGACAGCCTGCGTGTTTGGGTGTTCGGCGCAGGGACCATACT12530.10221045401671586No Hit
CCTTTACTAACTCTGTTGTGCAGTGGGTGCGACAGGCTCGTGGACAACGC12460.10163944589371744No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12300.10033428446972108No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)